Method for measuring quality of medicinal sodium lactate solution

A technology for measuring value and lactate oxidase, which is applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problem of inability to specifically determine the content of L-sodium lactate D-sodium lactate in sodium lactate solution, and achieve specificity. High, high analysis accuracy, stable baseline current effect

Inactive Publication Date: 2009-01-21
BIOLOGY INST OF SHANDONG ACAD OF SCI
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Problems solved by technology

Various existing chemical analysis methods including titration, colorimetry, and high performance liquid chromatography cannot specifically determine the content of L-sodium lactate or D-sodium lactate in sodium lactate solution

Method used

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  • Method for measuring quality of medicinal sodium lactate solution
  • Method for measuring quality of medicinal sodium lactate solution
  • Method for measuring quality of medicinal sodium lactate solution

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Embodiment Construction

[0029] 1 Instruments and reagents: D-lactate oxidase (EC 1.1.1.26): American Sigma Company; D-sodium lactate: American Sigma Company; glutaraldehyde: Shanghai Chemical Reagent Purchasing and Supply Station imported packaging; nuclear microporous membrane: American Nucleopore company (φ0.2μm); platinum and silver: Jinan Branch of the People's Bank of China (purity 99.999%); other reagents are analytically pure, medicinal sodium lactate solution (specification: sodium lactate not less than 40%, batch number: 070812, 070817 , 070928): A pharmaceutical factory in Wuxi. Instruments SBA-40 Lactic Acid Biosensing Analyzer (Shandong Academy of Sciences Institute of Biology).

[0030] 2 Determination principle: immobilized D-lactate oxidase combined with H 2 o 2 Electrode determination of D-sodium lactate principle

[0031]

[0032]

[0033]

[0034] Preparation of 3D-sodium lactate reference substance solution: Accurately weigh 50 mg of D-sodium lactate reference substance...

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Abstract

The invention relates to a method for detecting the quality of sodium lactate solution for medicine, which belongs to the technical field of biosensors. The process for preparation comprises combing D-lactate oxidase and a H2O2 electrode to form a D-lactalase electrode biosensor after D-lactate oxidase is solidified, adopting 0.1mmol.L-1 pH 7.2 phosphate buffer, wherein standard liquid contains D-lactic acid 500mg.L-1, automatically displaying the basal activity of enzyme membrane after standard liquid which is 25mul is injected in a reaction tank for 20s, pressing a calibration key (Calibrate) to calibrate, and measuring samples after automatically cleaning.

Description

[0001] Field [0002] The invention relates to a drug analysis and determination method. Specifically, the invention relates to a method for determining components in medicinal sodium lactate solution. Background technique [0003] Sodium lactate solution is prepared by neutralizing medicinal dilute lactic acid with sodium hydroxide (or sodium carbonate) solution (Chinese Pharmacopoeia Two Notes 1990: 358). [0004] Due to the chiral structure in the lactic acid molecule, and the process of fermentation production and post-treatment purification, purification, neutralization, etc., it has not been resolved by epirotation, so medicinal sodium lactate contains two kinds of D-sodium lactate and L-sodium lactate. optical isomers. [0005] The content determination under Chinese Pharmacopoeia sodium lactate solution item adopts acid-base titration method (Chinese Pharmacopoeia two 2005: 348), what record is the total content of D-sodium lactate, L-sodium lactate, can not measure ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/416
Inventor 史建国孙士青杨俊慧马耀宏李雪梅赵晓华孟庆军杨艳王丙莲张利群
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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