Method for producing Gypsophila seedling using mini-type cuttage

A technology of miniature cuttings and gypsophila, applied in the field of plant biology, can solve the problems of low survival rate and seedling rate of tall seedlings, long time for seedling supply, difficulty in mass production of seedlings, etc. The effect of improving the quality of seedlings and improving the survival rate of transplanting

Active Publication Date: 2009-02-04
YUNNAN YUNKE FLOWER
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the quality problems of "high-legged seedlings" existing in the existing Gypsophila cuttings and tissue culture, as well as the production problems of low survival rate and seedling rate, and also in order to solve the long and conce...

Method used

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  • Method for producing Gypsophila seedling using mini-type cuttage

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Experimental program
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Effect test

Embodiment 1

[0019] 1. Selection of explants and establishment of a sterile system: When selecting explants, select newly germinated and strong vegetative buds from plants with obvious variety characteristics and good flowering performance, remove the leaves, and cut off the 0.5cm terminal buds. Peel off the outer leaves, wash in water with a small amount of detergent, sterilize in 0.14% mercuric solution for 15 minutes, then sterilize in 0.2% sodium hypochlorite solution for 10 minutes, rinse twice with sterile water and inoculate Proliferation medium: MS+BA1.0mg / L+NAA0.1mg / L+agar 6.5g / L+sugar 30g / L culture medium, at a temperature of 24°C, light intensity of 2000Lx, light time of 12 hours / Under the condition of 1 day, proliferate and cultivate for 20 days, and then get clump-like seedlings;

[0020] 2. Subculture proliferation culture: cut the tufted seedlings obtained in step A into 1.5cm-long sections with 1-3 buds, and transfer them to MS+BA1.0mg / L+NAA0.1mg / L+agar 6.5 g / L+sugar 30g / ...

Embodiment 2

[0026] 1. Selection of explants and establishment of a sterile system: When selecting explants, select newly germinated and strong vegetative buds from plants with obvious variety characteristics and good flowering performance, remove the leaves, and cut off the 1.0cm terminal buds. Peel off the outer leaves, wash in water with a small amount of detergent, sterilize in 0.14% mercuric solution for 15 minutes, then sterilize in 0.2% sodium hypochlorite solution for 10 minutes, rinse twice with sterile water and inoculate Proliferation medium: MS+BA1.5mg / L+NAA0.1mg / L+agar 7.0g / L+sugar 40g / L culture medium, at a temperature of 26°C, light intensity of 2500Lx, light time of 10 hours / Under the condition of 1 day, cultured for 10 days;

[0027] 2. Subculture proliferation culture: cut the clump-like seedlings obtained in step A into 1.5cm-long sections with 1-3 buds, and transfer them to MS+BA1.5mg / L+NAA0.1mg / L+agar 7.0 g / L+white sugar 40g / L on the subculture medium, under the cond...

Embodiment 3

[0035] The selection of explants and the establishment of the sterile system were the same as in Example 1.

[0036] Induction of root primordia: Cut the subcultured seedlings into 1cm seedlings with shoot tips, inoculate them on the medium of MS+IBA0.3mg / L+agar 7.0g / L+sugar 30g / L, pH value 6.0, and culture The temperature is 26°C, the light intensity is 2500Lx, and the light time is 8 hours / day, and cultured for 6-7 days. After the root primordia are formed, micro-cuttings are carried out.

[0037] Miniature cuttage: above-mentioned rooted seedling is taken out from the bottle and soaked in 0.15% thiophanate-methyl solution for 1 minute, after taking out, the base of the seedling is stained with the root sun solution of 100ppm and cuttage is used as the seedling tray of the matrix with perlite, The substrate thickness is 2.5-3.0cm, and the cutting density is 1.0cm×1.5cm.

[0038] Management of cutting seedlings: with embodiment 1.

[0039] The transplanting of cutting seedl...

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Abstract

The invention provides a method for rapidly producing gypsophila seedlings by micro-cuttage. Tradable high-quality seedlings can be obtained by selecting explants, establishing a germfree system, performing subculturing and propagation, induction culture of root primordium, micro-cuttage, managing cottage seedlings, and transplanting the cottage seedlings. The method shortens seedling production time, prolongs seedling supply time and improves seedling quality. Compared with the prior art, the survival rate is increased by 6.2%, the survival rate of the fixed planting is increased by 5.8%, and the high-quality seedlings reaches over 85%, which is an economical, practical and efficient seedling production technology.

Description

technical field [0001] The invention relates to a rapid production method of cut flower gypsophila seedlings, in particular to a method for rapid production of gypsophila seedlings by miniature cuttings, and belongs to the field of plant biotechnology. Background technique [0002] Gypsophila is one of the most popular cut flowers in the world and one of the top ten cut flowers in Europe. In my country, it is mainly planted in Yunnan Province, with a planting area of ​​nearly 4,000 mu, and more than 3 million seedlings are needed every year. The production of Gypsophila in my country is mainly based on tissue culture and propagation. Some people have carried out experimental research and production of Gypsophila cuttings, but there are many technical problems in light and water management, and the survival rate and seedling rate of cuttings are not high. , and the root system of the seedlings is poor, and the survival rate of transplanting is low. Tissue culture seedling pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 吴丽芳屈云慧赵培飞杨春梅蒋亚莲张素芳李绅崇
Owner YUNNAN YUNKE FLOWER
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