Sheep hemoglobin blood pressure reduction peptide and preparation method

A technology of hemoglobin and blood pressure-lowering peptides, which is applied in food preparation, peptide/protein components, animal protein processing, etc., can solve the problem of undiscovered extraction of blood-pressure-lowering peptides, and achieves non-toxic side effects, low production costs, and high safety Effect

Inactive Publication Date: 2009-02-18
QINGDAO BOER PROSPEROUS MEAT PACKING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, no report about extracting blood pressure-lowering peptide from sheep hemoglobin was found

Method used

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  • Sheep hemoglobin blood pressure reduction peptide and preparation method
  • Sheep hemoglobin blood pressure reduction peptide and preparation method
  • Sheep hemoglobin blood pressure reduction peptide and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of Sheep Hemoglobin Antihypertensive Peptide

[0032] (1) 2g sheep hemoglobin dry powder (made by centrifugal spray drying of sheep blood) was dissolved in 100ml distilled water to obtain 2% sheep hemoglobin solution;

[0033] (2) Incubate the sheep hemoglobin solution at 70°C for 8min; then adjust the pH of the sheep hemoglobin solution to 7.8 with 1N HCL or NaOH, add 0.02g 2709 alkaline protease (produced by Beijing Donghua Qiangsheng Biotechnology Co., Ltd.), and then incubate at 52°C , pH7.8 under the condition of hydrolysis for 5h; to obtain sheep hemoglobin hydrolyzate,

[0034] (3) Filtrate the sheep hemoglobin hydrolyzate through a 3KDa hollow fiber ultrafiltration membrane (produced by Beijing Xucheng Ultrafiltration Equipment Factory) at 18°C ​​and a pressure of 0.15MPa, and then desalt through an ion exchange resin to obtain Sheep hemoglobin hydrolyzate solution;

[0035] (4) The goat hemoglobin hydrolyzate solution was incubated at 95° C. for 8...

Embodiment 2

[0038] Preparation of Sheep Hemoglobin Antihypertensive Peptide

[0039] (1) Get 5g sheep hemoglobin dry powder (according to conventional method, utilize sheep blood to make through centrifugal spray drying), be dissolved in 400ml distilled water, obtain 1.2% sheep hemoglobin solution;

[0040] (2) Sheep hemoglobin solution was incubated at 75°C for 6min, then adjusted to pH 8.0 with 1N HCL or NaOH, then added 0.04g 2709 alkaline protease (produced by Beijing Donghua Qiangsheng Biotechnology Co., Ltd.), and then heated at 55°C and pH8. Under the condition of 0.0, carry out the hydrolysis reaction for 6 hours to obtain sheep hemoglobin enzymatic hydrolyzate;

[0041](3) Filtrate the sheep hemoglobin enzymatic hydrolysis solution through a 5KDa hollow fiber ultrafiltration membrane (produced by Beijing Xucheng Ultrafiltration Equipment Factory) at a temperature of 20°C and a pressure of 0.12MPa, and the resulting filtrate is passed through an ion exchange resin. Desalting to o...

Embodiment 3

[0044] Composition Determination of Sheep Hemoglobin Antihypertensive Peptide of the Present Invention

[0045] Proceed as follows

[0046] (1) Determination of protein content

[0047] Weigh 0.1-0.3g or 3mL of the sample to be tested (Example 1 or Example 2) into a digestion tube, add 10mL H 2 SO 4 , 0.3g k 2 SO 4 , 0.03g CuSO 4 , placed on a digestion device for digestion, first digested at 250 ° C for about 1 hour, until no carbon particles popped out, and then gradually warmed to 500 ° C to digest until transparent and light blue or light green, and then with automatic Kjeldahl Nitrogen distillation and titration. The protein content is:

[0048]

[0049] In the formula: V is the milliliter number (mL) of consumed hydrochloric acid solution during titration; N is the equivalent concentration (mol / L) of standard hydrochloric acid solution; 0.014 is the gram number of 1mg equivalent nitrogen; 6.38 is the protein conversion coefficient of nitrogen; W is the sample ...

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Abstract

The invention discloses a sheep hemoglobin blood pressure reduction peptide and a method for preparing the same. The method comprises the following steps: sheep hemoglobin dry powder is dissolved in distilled water and kept warm at a temperature of between 70 and 80 DEG C for 5 to 10 minutes, and the sheep hemoglobin solution is added with alkali protease to perform the hydrolysis reaction at a temperature of between 50 and 60 DEG C and a pH value of between 7.5 and 8.5 for 4 to 6 hours, and the obtained sheep hemoglobin enzymolysis solution is subjected to ultrafiltration, desalting, and centrifugal spray drying, finally the sheep hemoglobin blood pressure reduction peptide is obtained. The blood pressure reduction peptide has the advantages that: firstly, the blood pressure reduction peptide has strong capability of reducing blood pressure, of which the IC50 value is between 0.78 and 0.86mg/mL, and the ACE inhibition activity reaches 92 to 95 percent; secondly, the blood pressure reduction peptide only takes effect of pressure reduction on a hypertension patient, so that the over-reduction problem of blood pressure is not existed; thirdly, the blood pressure reduction peptide is extremely high in safety and has no toxic and side effects; and fourthly, the preparation method is low in cost. The sheep hemoglobin blood pressure reduction peptide can also be used as a nutrient health product.

Description

technical field [0001] The invention belongs to the field of health care medicine or functional food, and in particular relates to a goat hemoglobin hypotensive peptide and a preparation method thereof. Background technique [0002] Hypertension is the most common cardiovascular disease. According to World Health Organization (WHO) estimates, the number of patients worldwide has exceeded 500 million. More than 95% of the hypertensive population has essential hypertension, and the essential hypertension can be effectively reduced by inhibiting the enzyme activity of angiotensin I converting enzyme (ACE). Although the artificially synthesized ACE inhibitors can effectively reduce high blood pressure, there are many clinical adverse reactions. Therefore, it is a good way to achieve the purpose of curing and preventing diseases through non-drug methods. Angiotensin Iconverting enzyme inhibitory peptides (ACEIP for short) derived from food protein is a kind of polypeptide substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/12A23L1/305A61K38/02A61P9/12A23L33/17
Inventor 于雪初罗永康
Owner QINGDAO BOER PROSPEROUS MEAT PACKING
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