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Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein

A technology of milk casein and inhibitory peptides, which is applied in the field of step-by-step enzymatic hydrolysis of goat milk casein to prepare ACE inhibitory peptides, achieving mild properties and promoting digestion and absorption

Inactive Publication Date: 2013-07-24
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the microbial fermentation method, the enzymatic reaction is fast, and the preparation of ACE inhibitory peptides has a high inhibitory activity, and the raw materials are widely used, mainly including milk protein, plant protein and animal protein. However, at present, milk-derived ACE inhibitor peptides are mainly made of cow milk. , has not been involved in goat milk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1: Mix and dissolve goat milk powder and water at a mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, centrifuge at 6400rpm for 15 minutes with a high-speed centrifuge, remove fat, and obtain skim milk;

[0028] Step 2: heat the obtained skim milk in a constant temperature water bath at 35°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution is 4.6, Precipitate the protein, centrifuge at 6400rpm for 15min with a high-speed centrifuge, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 12h, and then transfer to a freeze dryer at -55°C Freeze-dried at low temperature for 24 hours to obtain freeze-dried casein powder; when the temperature of skim milk was 35°C, the pH value was 4.6, and the stirring intensity was 800 rpm, the casein yield was the highest, reaching 9.23%.

Embodiment 2

[0030] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, centrifuge at 6400rpm for 15min with a high-speed centrifuge, remove fat, and obtain skim milk;

[0031]Step 2: Insulate the obtained skim milk in a constant temperature water bath within the range of 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.5, Precipitate the casein, centrifuge at 6400rpm for 15min with a high-speed centrifuge, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 12h, and then transfer to a freeze dryer at - Freeze-dry at 55°C for 24 hours to obtain freeze-dried casein powder; when the skim milk is at 45°C, pH is 4.5, and stirring intensity is 800rpm, the yield of casein is the highest, reaching 11.88%.

[0032] Step 3: Mix the obtained freeze-dried casein powd...

Embodiment 3

[0035] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;

[0036] Step 2: heat the obtained skim milk in a constant temperature water bath at 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution is 4.5, Precipitate the protein, centrifuge at 6400rpm for 15min with a high-speed centrifuge, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 12h, and then transfer to a freeze dryer at -55°C Freeze-dried at low temperature for 24 hours to obtain freeze-dried casein powder; when the temperature of skim milk was 45°C, the pH value was 4.5, and the stirring intensity was 800 rpm, the yield of casein was the highest, reaching 11.88%.

[0037] Step 3: Mix the o...

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Abstract

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through substep enzymatic hydrolysis of feta protein. The method comprises the following steps of: cooling and centrifuging recovered feta for degreasing to obtain skimmed milk, using the skimmed mild for preparing casein by isoelectric precipitation, firstly hydrolyzing the casein with Alcalase to obtain hydrolysate containing the ACE inhibitory peptides through optimization of hydrolysis condition, and performing optimization of neutral protease and trypsin hydrolysis conditions by taking the hydrolysate as a substrate to obtain small-molecule peptides with high ACE inhibitory activity through. The inhibitory proportion of an ACE inhibitory peptide product prepared by the method can reach above 90%; and the ACE inhibitory peptide product has the characteristics of being diversified in variety of the ACE inhibitory peptides, having small fragments, being beneficial to the separation and purification of the small-molecule peptides and being high in inhibitory proportion.

Description

technical field [0001] The invention relates to a step-by-step enzymolysis method for preparing ACE inhibitory peptide from goat milk casein. Background technique [0002] Hypertension is one of the most common cardiovascular diseases, which often causes heart, brain and kidney complications, and is the main risk factor for stroke and coronary heart disease. Hypertension refers to the condition of systolic blood pressure ≥ 140mmHg and (or) diastolic blood pressure ≥ 90mmHg without the use of antihypertensive drugs. According to the latest statistics from the National Hypertension Research Center, there are 120 million hypertensive patients in my country, and the incidence rate is 10.8%. According to the forecast of the World Health Organization, by 2020, non-communicable diseases will account for 79% of the causes of death in my country, of which cardiovascular diseases will take the first place. For every 5mmHg reduction in systolic blood pressure, the risk of cardiovascu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 陈合王娟舒国伟
Owner SHAANXI UNIV OF SCI & TECH
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