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Special effect phosphate accumulating organisms and waste water processing method using thereof

A technology of phosphorus accumulating bacteria and special effects is applied in the field of special phosphorus accumulating bacteria and its treatment of wastewater, achieving the effects of low cost, no secondary pollution and stable efficiency.

Inactive Publication Date: 2012-07-04
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual operation, these processes have problems such as slow start and recovery of the phosphorus removal system, and unstable phosphorus removal efficiency. When the organic matter content in the wastewater is low, or the phosphorus content exceeds 10mg / L, the effluent is difficult to meet the phosphorus discharge standard

Method used

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  • Special effect phosphate accumulating organisms and waste water processing method using thereof
  • Special effect phosphate accumulating organisms and waste water processing method using thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Breeding of high-efficiency phosphorus-accumulating strains and determination of phosphorus-accumulating rate

[0028] (1) Collect samples

[0029] The mud samples from the bottom of Buji River in Shenzhen City were collected on site in a 500ml sterile triangular flask, and after adding distilled water, they were shaken to pieces with glass strains.

[0030] (2) Strain enrichment and isolation

[0031] Take 10 ml of the sample solution into a Erlenmeyer flask filled with 150 ml of enriched medium (sterilized), and culture it on a shaker at 30° C. and 150 r / min for 3 days. Enrichment medium components: anhydrous sodium acetate 5.0g, MgSO 4 .7H 2 O 0.5g, CaCl 2 0.2g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 2-20mg, trace elements 1ml, distilled water to 1000ml, pH value 7.2-7.4, after the medium becomes turbid, then transfer 8ml to a new enrichment medium, shake culture for 3 days under the same conditions, and repeat this 4 times , each transfer volume is: ...

Embodiment 2

[0038] Example 2: Pilot test of HJP07 strain on phosphorus removal efficiency of river sewage

[0039] The experimental process adopts biofilm-activated sludge composite treatment process. The sewage is taken from the sewage of Buji River in Shenzhen, with a total phosphorus content of 1-6mg / L. The inoculated sludge is taken from the SBR reactor of the urban sewage treatment plant, and the system is operated aerobically. The HJP07 strain was inoculated in the enrichment medium of a 300mL Erlenmeyer flask (phosphorus concentration 10mg / L), cultivated on a shaker at 30°C and 150r / min, and measured its bacterial cell concentration by plate counting method at the end of the logarithmic growth phase. 10 8 For CFU / L, add a certain volume of bacterial solution to the reactor to make the bacterial concentration 5×10 6 CFU / L, first aerate the system for 48 hours, then run the reactor in an aerobic mode, and measure the total phosphorus content in the effluent. The test found that the H...

Embodiment 3

[0040] Example 3: Application effect of HJP07 strain in Shenzhen Buji River sewage treatment practice project

[0041] Buji River Water Quality Evolution Plant adopts process: enhanced submerged biofilm-activated sludge (SBF-AS) composite biological treatment process (EHYBFAS process, patent number: 200620017991.5). After the HJP07 bacterial strain is cultivated in the laboratory according to the method of Example 2, take 5% of the inoculum to ferment in a 5000L sterilized culture tank, and the culture tank is equipped with an adjustable-speed stirrer and microporous aeration with adjustable aeration device, cultured to a cell concentration of 10 8 ~10 9 CFU / L. When the system is started, a certain amount of bacterial solution is added to the aerobic section of the SBF-AS reaction tank to accelerate the start of the system. During the operation of the system, when the concentration of phosphorus accumulating bacteria in the reaction tank is lower than 10 4 When CFU / L, add a...

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Abstract

The invention discloses a specific phosphorus-accumulating bacterium. The strain is a phosphorus-accumulating bacterial strain HJP07, wherein the preserving number of the bacterial strain is CGMCC No. 2164 and the preserving date of the bacterial strain is September 13th, 2007. The phosphorus-accumulating bacterial strain is separated from sludge, cultured in an enriched medium and separated in a separation culture medium, wherein the pH value of the phosphorus-accumulating bacterium at a temperature of between 25 and 35 DEG C is between 6.5 and 8.0. By adopting the phosphorus-accumulating bacterium to treat waste water, the cost is low, the efficiency is stable and no secondary pollution produces, thereby effectively treating phosphor-containing waste water and protecting the environment.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a special-effect phosphorus-accumulating bacterium and a method for treating waste water. 【Background technique】 [0002] Phosphorus is the most important factor of water eutrophication, so the removal of phosphorus in water is particularly important to prevent water eutrophication. Looking at domestic sewage treatment plants, phosphorus removal technology has always been a problem that plagues the operation of sewage treatment plants. The traditional physical and chemical phosphorus removal technology requires a large amount of chemicals, which has the disadvantages of high operating costs and large sludge output. Although most of the existing urban sewage treatment plant processes include denitrification and phosphorus removal, through the improvement of the design of the sewage biological treatment system or the change of the operation mode, the phosphorus accumulating bacteri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/39
Inventor 金文标闫韫赵勇娇陈亚松
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL