Method for preparing natamycin

A technology of natamycin and Streptomyces lidi, which is applied in the field of preparing natamycin and can solve the problems of ineffectiveness of bacteria and viruses

Active Publication Date: 2009-04-01
中农绿康(北京)生物技术有限公司
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Natamycin is resistant to almost all molds and yeasts, and its antibacterial effect is about 50 times stronger than that of sorbic aci...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing natamycin
  • Method for preparing natamycin
  • Method for preparing natamycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the isolation of Streptomyces lydicus (Streptomyces lydicus) A01 CGMCC No.1653

[0059] Collect soil samples from vegetable fields in Miyun County, Beijing, take 10g and add them to a triangular flask containing 100ml sterile water and appropriate amount of glass beads, shake on a shaker at 100rpm for 20min, take 0.5ml and add them to 4.5ml sterile water, and then dilute for 10 2 、10 3 、10 4 times, take 0.1ml of the above soil suspension and evenly spread it on the Gaoshi No. 1 medium plate, and blow it in the ultra-clean workbench until it is slightly dry; each concentration is repeated 3 times, and cultured in a 28°C incubator for 5-10 On the first day, a single colony of actinomycetes was picked for dilution, streaking, isolation and purification to obtain a pure culture strain.

[0060] Primary screening: the obtained pure culture strains were subjected to shake-flask fermentation, and the fermentation broth was filtered through a 0.45 μm sterile mic...

Embodiment 2

[0078] Embodiment 2, utilize Streptomyces lydida shake flask fermentation to produce natamycin

[0079] Streptomyces lydidis A01 CGMCC No.1653 and A02 CGMCC No.1654 can produce a large amount of biologically active substances in the medium during the metabolic process. The biologically active substance has a strong inhibitory effect on the indicator strains Saccharomyces cerevisiae ACCC20036, Aspergillus niger ACCC30005 and Botrytis cinerea ACCC30091; its paper chromatogram of the Czech eight-solvent system is consistent with that of polyene antibiotics; its ultraviolet spectrum shows tetraenes Typical absorption peak unique to antibiotics, which is consistent with the results of Czech eight-solvent system paper chromatography; after preliminary separation, it is found that there are 2 active components, and after the main active components are purified, ultraviolet spectroscopy (UV), Infrared spectrum (IR), mass spectrum (MS) and nuclear magnetic resonance spectrum (NMR) dete...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for preparing natamycin, which carries out fermentation to Streptomyces lydicus so as to obtain the natamycin. The method initiatively uses the fermentation of the Streptomyces lydicus for producing the natamycin, thus providing a new way for producing the natamycin. The natamycin produced by the method has high purity. The method can be widely applied to the production of the high-purity natamycin.

Description

technical field [0001] The invention relates to a method for preparing natamycin. Background technique [0002] Natamycin (Natamycin) is a polyene macrolide antifungal antibiotic, also known as natamycin or Pimaricin (Pimaricin), which is a multi-enzyme system encoded by five polyacetyl synthetase genes Synthetic, capable of obligately inhibiting yeasts and moulds, preventing the formation of aflatoxins in filamentous fungi. Its actual dosage is 10 -6 Order of magnitude, with the characteristics of low dosage, high efficiency, and long antibacterial action time, it is an efficient and safe natural biological food preservative and antibacterial additive, which has been widely used in more than 30 countries, including the European Union, most of North America and Eastern European countries, some Middle Eastern countries, and even Switzerland, which attaches great importance to food safety, allow the use of natamycin in bread and cheese products. In 1996, my country's Food A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/08C12R1/465
Inventor 刘伟成裘季燕刘建华卢彩鸽刘霆刘德文隋勤
Owner 中农绿康(北京)生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap