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Flow assay device comprising dry reagent cake

An analysis device and flow analysis technology, which is applied in the direction of material analysis, measurement device, and analysis materials through observation of the influence of chemical indicators, can solve the problems of unsuitability for continuous flow displacement analysis and achieve high analysis results.

Inactive Publication Date: 2009-04-22
梁贵合
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Visually identifiable labels are usually large molecules or aggregated small molecular particles, not suitable for use in continuous flow displacement assays

Method used

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  • Flow assay device comprising dry reagent cake
  • Flow assay device comprising dry reagent cake
  • Flow assay device comprising dry reagent cake

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Experimental program
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Embodiment Construction

[0029] according to figure 1, the components of the flow analysis device 100 of the present invention include: a first reagent chamber 110, the first reagent chamber has a soluble reagent piece 111 composed of a labeled reagent 112; an immobilized reagent part 120 has an immobilized reagent 121; and a liquid receiving part 130 . The adjoining portion is capable of fluid communication. The sample solution can be guided to the first reagent chamber 110 , dissolve the reagent block 111 , flow through the immobilized reagent part 120 to the solution receiving part 130 . During the flow, the analyte in the sample solution, the labeled reagent 112 of the reagent block 111 and the immobilized reagent 121 of the immobilized reagent portion 120 contact each other to initiate an analytical reaction involving the analyte and the reagent. As a product of the analysis reaction, a portion of the labeled reagent 112 is bound to the immobilized reagent portion, and the unbound labeled reage...

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Abstract

The current invention provides an assay device for detecting an analyte in a sample solution comprising a first reagent chamber having a soluble reagent cake comprising a labeled reagent in fluid communication with an immobilized reagent section having an immobilized reagent, which is further in fluid communication with a fluid receiving section. The assay method using the device of the invention comprises applying a sample solution to the first reagent chamber, dissolving the reagent cake, and flowing the sample solution comprising the dissolved labeled reagent through the immobilized reagent section to the fluid receiving section of the device. The assay result is determined by reading a signal of the label of the labeled reagent at the immobilized reagent section or the fluid receiving section. The device preferably comprises a flow control mechanism between the first reagent chamber and the immobilized reagent section.

Description

technical field [0001] The present invention relates to a novel analytical reagent component, an analytical device and a method for diagnostic testing of sample solutions. In particular, the invention relates to flow analysis devices containing soluble lyophilized reagent pellets. Background technique [0002] An analyte binding reaction based flow assay device for detecting an analyte in a sample solution consists of a labeling reagent, a liquid permeable solid matrix, and a liquid receiving portion, the liquid permeable solid matrix consisting of an immobilized reagent portion with an immobilized reagent composition. A labeling reagent is an analyte analog or an analyte conjugate labeled with a detectable label. Immobilization reagents, analyte conjugates, or analyte analogs are immobilized on the surface of a solid substrate. The combination of the labeled reagent and the immobilized reagent is configured for a competition assay reaction or a sandwich assay reaction, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00G01N21/78G01N21/86G01N33/558
CPCG01N33/558G01N21/8483G01N21/78G01N33/54388
Inventor 梁贵合
Owner 梁贵合