A kit for predicting the risk of high altitude pulmonary edema by the copy number of agt gene

Abstract

The invention relates to a kit for predicting the incidence risk of high altitude pulmonary edema through the copy number of AGT gene, which comprises separated and packaged AGT primer mixture, Beta-actin primer mixture, PCR reaction solution, sterile deionized water, and fixed-value standards , healthy control DNA, characterized in that the sequences of the upstream and downstream primers in the AGT primer mixture are: F: 5'-GCCGTTTCTCCCTTGGTCT-3' and R: 5'-GCAAGACGTTTATTACTAACACAAG-3'; The sequences of the upstream and downstream primers in the Beta-actin primer mixture are: F: 5'-CGGGAAATCGTGCGTGACAT-3' and R: 5'-GAAGGAAGGCTGGAAGAGTG-3'. The invention can be used to screen people who are susceptible to high altitude pulmonary edema before plain people enter the plateau, guide the prevention and treatment of high altitude pulmonary edema, and benefit the health of people entering the plateau; the kit is simple to use, convenient to operate and fast to detect.

Description

technical field [0001] The invention relates to a detection kit, in particular to a kit for predicting the incidence risk of high altitude pulmonary edema through the copy number of AGT gene, which is used for evaluating the susceptibility of human high altitude pulmonary edema. Background technique [0002] High altitude pulmonary edema (HAPE for short) is a type of pulmonary edema that is unique to high altitude hypoxic environments. Respiratory failure, even death, is an acute severe altitude sickness that seriously threatens the health and life of people entering the plateau. Surveys in recent years have shown that the incidence of high altitude pulmonary edema is as high as 0.4%-2% in the Han population who entered the plateau above 3000 meters above sea level from the plain. The incidence of high-altitude pulmonary edema has obvious family and individual susceptibility tendencies, and both environmental and genetic factors can affect the occurrence of high-altitude pu...

AI Technical Summary

Problems solved by technology

At present, it has been reported in the literature that the copy number of some genes is associated with the susceptibility to diseases and is a genetic marker of some diseases [Braude I, Large scale copy number variation (CNV) at 14q12 is associated with the presence of genomic abnormalities in neoplasia, "BMC Genomics", 20067: 138.], there is no report on the correlation between AGT copy number and susceptibility to high altitude pulmonary edema