Use of terramycin in preparing medicine for treating aquatic animal spiroplasmosis

A technology for aquatic animals and spiroplasmosis, which is applied in the directions of drug combination, anti-infective drugs, active ingredients of tetracycline, etc., to reduce economic losses and achieve significant effects.

Active Publication Date: 2011-07-20
常熟紫金知识产权服务有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Oxytetracycline has been widely used in the field of aquaculture. It is one of the six drugs approved by the US FDA in 2006 for aquaculture. It can be used to treat vibriosis and enteritis in fish, but it has not been reported for the treatment of Diseases caused by spiroplasma

Method used

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  • Use of terramycin in preparing medicine for treating aquatic animal spiroplasmosis
  • Use of terramycin in preparing medicine for treating aquatic animal spiroplasmosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: drug susceptibility test

[0015] (1) Configuration of liquid medicine:

[0016] Preparation of quicklime solution: Weigh 2 grams of Cao and dissolve it in 100ml sterilized double-distilled water to prepare a 20000 mg / L suspension medicinal solution, and use sterilized tap water to perform gradient dilution by doubling dilution method to prepare various series of concentration group medicinal solutions. The solution preparation method of Qingtangjing, dimethilone, copper sulfate, bromochlorohydantoin, and potassium permanganate is the same as that of quicklime. Preparation of penicillin and oxytetracycline liquid: Weigh 100mg and dissolve in 100mL absolute ethanol respectively to make 1000mg / L liquid, use R2 culture medium to carry out gradient dilution by doubling dilution method, and make each series concentration group containing drug culture base. Preparation of Chinese gallnut and yellow lotus liquid medicine: Weigh 5g of Chinese gallnut and yellow ...

Embodiment 2

[0029] Embodiment 2: the acute toxicity test of oxytetracycline to Chinese mitten crab

[0030] Experimental procedure:

[0031] Eriocheir sinensis was first raised in the laboratory for 3 days, with an average body weight of 5.5g. The temperature was set at 27°C. And use the random sampling method to carry out light microscope inspection to ensure that it is healthy Chinese mitten crab without spiroplasma infection.

[0032] Find out the concentration range of 0~100% mortality within 24 hours according to the preliminary test, and set up 6 concentration groups (50mg / ml, 20mg / ml, 10mg / ml, 5mg / ml, 2.5mg / ml, 0.5 mg / ml), a blank control group and a negative control group. The test drug solution was prepared from the mother solution, and then gradually diluted according to the requirements to make the required concentration of drug solution. Chinese mitten crabs were divided into 8 groups, 40 in each group, and six groups were injected sequentially according to the selected con...

Embodiment 3

[0034] Embodiment 3: the relapse treatment test of oxytetracycline

[0035]First, the Chinese mitten crabs are taken back and temporarily raised in the laboratory for 3-4 days. The average weight is 50g / only. According to the law of shivering disease, the temperature was set at 27°C. The healthy Chinese mitten crabs without spiroplasma were ensured by light microscope inspection by random sampling method. Set up 6 experimental groups, one echo control group and one negative control group, with 20 rats in each group. The purely cultured spiroplasma strains were used to reinfect the experimental group and the control group of Chinese mitten crabs, each reinfected with 100 microliters of bacterial liquid, and the negative control group was reinfected with the same volume of blank medium. After 24 hours, oxytetracycline injection was given. The therapeutic concentrations of oxytetracycline are 50mg / mL, 20mg / mL, 10mg / mL, 5mg / mL, and 2.5mg / mL, respectively. Due to the high temp...

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Abstract

The invention discloses the application of terramycin in preparing a medicament for curing aquatic spiroplasma diseases. The terramycin is used for curing the diseases caused by the spiroplasma, particularly the shaking diseases of eriocheir sinensis; therefore, the invention provides an effective approach for controlling the aquatic diseases; moreover, the effect is remarkable and the cure rate can be more than 80 percent.

Description

technical field [0001] The invention relates to a new application of oxytetracycline, in particular to the application of oxytetracycline in the preparation of medicines for treating aquatic animal spiroplasmosis. Background technique [0002] Aquatic animal spiroplasmosis refers to aquatic animal diseases caused by spiroplasma infection, such as shivering disease of Chinese mitten crab, outbreak of Penaeus vannamei and epidemic of Procambarus clarkii. Chinese mitten crab (Eriocheir sinensis), also known as river crab, is a special aquaculture species in my country and has extremely high economic value. "Shivering disease" is a serious epidemic that threatens the sustainable development of river crab aquaculture in my country. Shivering disease is an explosive disease with a high mortality rate. In some places, the incidence rate is as high as 70% and the mortality rate is over 90%. The water body even leads to extinction, which has caused great losses to river crab farming....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/65A61P31/00
Inventor 王文梁廷明吴霆顾伟封琦
Owner 常熟紫金知识产权服务有限公司
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