Compositions and methods for enzymatic decolorization of chlorophyll

A technology of chlorophyllase and chlorophyll, which is applied in the field of plant and animal products and enzymology, and can solve the problems of reducing oil production and increasing processing costs

Inactive Publication Date: 2009-05-13
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This bleaching step increases processing costs and reduces oil yield due to entrainment in the bleaching earth

Method used

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  • Compositions and methods for enzymatic decolorization of chlorophyll
  • Compositions and methods for enzymatic decolorization of chlorophyll
  • Compositions and methods for enzymatic decolorization of chlorophyll

Examples

Experimental program
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Embodiment 1

[0571] The following examples describe exemplary esterase (chlorophyllase activity) assays for isolating and characterizing enzymes of the invention and nucleic acids encoding them, and for determining whether a polypeptide is within the scope of the invention.

[0572] Screening of esterase activity on chlorophyll from spinach to produce chlorophyll. In this exemplary esterase (chlorophyllase activity) assay, the esterase screening format included:

[0573] This esterase screening method uses HPLC to analyze the reaction products. Figures 2 and 3 show data showing the results of assays for esterase (chlorophyllase) activity using the indicated exemplary enzymes of the invention.

[0574] The data presented in Figure 2 illustrate that between the 24 hour and 48 hour time points, the level of the reaction product increased, and the level of the reaction product indicated SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 6, SEQ ID NO: Chlorophyllase activity of ID NO:8, SEQ...

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Abstract

The invention provides compositions and methods for the enzymatic treatment (''bleaching'' or ''de-colorizing'') of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil.In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.

Description

technical field [0001] The invention relates to the industrial processing of food, feed or vegetable oils, plant and animal products and the fields of enzymology. In particular, the present invention provides compositions and methods for the enzymatic treatment ("bleaching" or "decolorization") of chlorophyll-containing compositions or chlorophyll-contaminated compositions which For example, algae, animal or plant preparations, food, feed or oils, such as vegetable oils, including oils processed from oilseeds such as rapeseed (rapeseed) oil or soybean oil, or oils processed from oilseeds such as palm oil. In one aspect, the invention provides a method of enzymatically modifying chlorophyll, eg, in algae, animal or plant preparations, or food, feed or oil using a chlorophyll catabolizing enzyme (eg, chlorophyllase). Background technique [0002] Vegetable oils from oilseeds such as canola or soybean, or from oilseed fruits such as palm, contain chlorophyll. Chlorophyll is r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04C12N9/06A23L5/40A23L5/41A23L27/21
CPCC11B3/003C12N9/18A23L1/277C12Y301/01014A23K1/1656A23K10/14A23L5/49
Inventor D·拉姆D·韦纳T·希契曼N·R·巴顿M·J·伯克
Owner DSM IP ASSETS BV
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