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Use of ursolic acid in preparing medicine capable of regulating and controlling tumour cell division cycle

A tumor cell, division cycle technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems such as limited application and high toxicity

Inactive Publication Date: 2009-06-03
YICHUN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These inhibitors are very toxic, which limits their clinical application

Method used

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  • Use of ursolic acid in preparing medicine capable of regulating and controlling tumour cell division cycle
  • Use of ursolic acid in preparing medicine capable of regulating and controlling tumour cell division cycle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction and separation of ursolic acid

[0040] Materials and Methods

[0041] Materials Loquat leaf medicinal residue is the waste of loquat syrup produced by water decoction. It is small strip-shaped and black in color. It is provided by Jiangxi Haiersi Pharmaceutical Company.

[0042] Reagent edible grade 95% ethanol (produced by Guangxi Sugar Factory); "YCXY21" impurity remover (self-made), activated carbon, analytically pure (produced by Shanghai Haoshen Chemical Reagent Co., Ltd.); Provided).

[0043] Instruments FC2204 analytical balance (Shanghai Balance Analytical Instrument Factory); Perkin Elmer Fourier transform infrared spectrometer (USA).

[0044] method

[0045] process flow. The new process of "alcohol extraction and condensation" is used for extraction and separation.

[0046] Loquat leaf dregs → add ethanol to reflux extraction → recover ethanol → remove impurities with impurity remover → decolorize with activated carbon → condensate separation...

Embodiment 2

[0056] Experimental materials (the following cell lines were purchased from the American ATCC Standard Cell Bank)

[0057]

[0058] main reagent

[0059] RPMI1640 medium dry powder GIBCO company

[0060] Calf serum GIBCO Company / Hangzhou Sijiqing Company

[0061] Trypsin, DMSO, MTT Fluka

[0062] Penicillin, Streptomycin Sigma

[0063] Ursolic acid standard substance, Jiangxi Provincial Key Laboratory of Natural Medicine Research, Yichun University

[0064] Cisplatin Jiangsu Senhao Pharmaceutical Co., Ltd.

[0065] Media and common solutions

[0066] Tumor cell medium: RPMI1640 medium added with 10% calf serum. Contains 2g / L NaHCO 3 , penicillin 100U / ml and streptomycin 100U / ml, filter sterilized, and store at 4°C.

[0067] PBS: Dissolve 0.2g KCl, 0.44g Na in 800ml water 2 HPO 4 and 0.24g KH 2 PO 4 , adjusted the pH to 7.4 with HCl, added water to make up to 1 L, and sterilized by autoclaving.

[0068] Digestive solution: EDTA / PBS (Ca-free 2+ Mg 2+ ) / trypsin ...

Embodiment 3

[0091] Regulation of UA on tumor cell division cycle

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Abstract

The invention belongs to the technical field of pharmacochemistry and discloses a use of ursolic acid in preparing medicine capable of regulating and controlling tumour cell division cycle. Experiments indicate that the ursolic acid can change the division cycle of lung cancer cell, gastric cancer cell, liver cancer cell, breast cancer cell and leukemia cell, and obviously control the cell division of the lung cancer cell, gastric cancer cell, liver cancer cell, breast cancer cell and leukemia cell. The ursolic acid can be used for preparing medicine capable of restraining the cell division of the tumour cell.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry. The invention discloses the use of ursolic acid in the preparation of drugs for regulating the division cycle of tumor cells. Experiments show that ursolic acid can change the division cycle of lung cancer, gastric cancer, liver cancer, breast cancer, and leukemia cells, and significantly inhibit lung cancer, gastric cancer, and liver cancer. , Cell division of breast cancer, leukemia cells. Ursolic acid can be used to prepare drugs that inhibit tumor cell division. Background technique [0002] Ursolic acid (UA), also known as ursolic acid and ursolic acid, is a pentacyclic triterpenoid compound. According to incomplete statistics, ursolic acid has been found in 108 species of plants in 34 families in nature, such as Scrophulariaceae The leaves of the plant Paulownia tomentosa, the leaves and fruits of the rhododendron bearberry, etc. Ursolic acid is a fat-soluble substance, which...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P35/00
Inventor 陆云华
Owner YICHUN UNIVERSITY
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