4 results about "Cell division cycle" patented technology

The present application relates to substituted pyrrole carboxamide derivatives of Formula (I) that modulate the activity of the cell division cycle 7-related kinase (Cdc7). Accordingly, the compounds of the present application are useful in the treatment of diseases associated with dysregulated kinase activity, such as cancer, cell proliferative disorders, viral infections, immune disorders, neurodegenerative disorders, cardiovascular diseases, and bone-related diseases. The present application also provides methods for making these compounds, pharmaceutical compositions comprising these compounds, and their medical uses.

The invention relates to the technical field of fluorescence detection, in particular to a cell nucleus targeting yellow fluorescent probe and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted thio-piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 6 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and a target product is obtained; the yellow fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus is obtained. The method can be applied to real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.

The invention relates to a combination therapy. In particular, a Cell Division Cycle 7 kinase inhibitor (CDC7i) used in a combination therapy with: i) a Cyclin Dependent Kinase 8 inhibitor (CDK8i), and / or ii) a Cyclin C inhibitor (CCNCi); for use in the treatment or prevention of cancer in a subject.

The invention relates to the technical field of fluorescence detection, in particular to a high-brightness fluorescent probe capable of rapidly and specifically staining cell nucleuses and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 2 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and the high-brightness fluorescent probe capable of rapidly and specifically staining the cell nucleuses is obtained. The green fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus within 1 minute is obtained. The method can be applied to rapid real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.