8 results about "Cell division cycle" patented technology

The invention relates to the technical field of biology, in particular to desmosome-XVII type collagen fusion protein as well as a preparation method and application thereof. The invention provides a recombinant XVII type collagen fusion protein which comprises at least one XVII type collagen functional domain fragment and at least one functional domain fragment of desmosome core glycoprotein, the recombinant human X VII type collagen fusion protein provided by the invention has better cell adhesion activity and cell proliferation promoting activity. The recombinant human XVII type collagen fusion desmosis protein can significantly improve the adhesion between cells in a cell culture environment, and compared with commercial human XVII type collagen, the recombinant human XVII type collagen fusion desmosis protein can improve the effect by several times or more. In the aspect of promoting cell proliferation, the cell division cycle can be effectively shortened, so that the number of cells is rapidly increased within the same time, and the XVII type collagen is remarkably superior to commercial human XVII type collagen, and has wider application potential in the fields of skin care products, cosmetics, medicines, biomedical materials and the like.

The invention provides a method for enhancing gene knock-in efficiency mediated by a CRISPR gene editing system in a cell. The method comprises the step of enabling the cell to be in contact with a DNA-dependent protein kinase (DNA-PK) inhibitor and a cell division cycle protein 7 (Cdc7) inhibitor in the gene knock-in operation process of the cell. The invention also provides a kit for enhancing the gene knock-in efficiency mediated by the CRISPR gene editing system in cells. According to the method and the kit provided by the invention, the DNA-PK inhibitor and the Cdc7 inhibitor are combined for use, so that the gene knock-in efficiency can be remarkably improved under the conditions of different cell lines, different insertion sites and different donor template lengths.

The present application relates to substituted pyrrole carboxamide derivatives of Formula (I) that modulate the activity of the cell division cycle 7-related kinase (Cdc7). Accordingly, the compounds of the present application are useful in the treatment of diseases associated with dysregulated kinase activity, such as cancer, cell proliferative disorders, viral infections, immune disorders, neurodegenerative disorders, cardiovascular diseases, and bone-related diseases. The present application also provides methods for making these compounds, pharmaceutical compositions comprising these compounds, and their medical uses.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a compound capable of combining with cell division cycle 20 (Cdc20) and a composition thereof, and particularly discloses a compound capable of combining with cell division cycle 20 (Cdc20), wherein the compound is shown as (I); the compound can further become a targeted protein degradation agent and a pharmaceutical composition, and is used for treating or preventing diseases caused by promoting abnormal cell division after Cdc20 activates an APC complex, and the compound has high Cdc20 combining ability.

The invention relates to the technical field of fluorescence detection, in particular to a cell nucleus targeting yellow fluorescent probe and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted thio-piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 6 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and a target product is obtained; the yellow fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus is obtained. The method can be applied to real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.

The invention relates to a method for regulating and controlling the cell division cycle to strengthen the electron transfer capability of shewanella. In order to explore the relationship between the cellular morphology and the electron transfer capability of electroactive microorganisms, a synthetic biology strategy is used for carrying out cellular morphology transformation on mode electrogenic bacteria shewanella, and four engineering strains capable of shortening the cell division cycle of shewanella are constructed. Morphological characterization shows that the cell size of the overexpression division protein engineering strain is reduced, and electrochemical characterization shows that the output voltage, current and power density of the engineering strain are gradually increased along with the reduction of the cell volume, which indicates that the overexpression division related protein can shorten the division cycle and accelerate the cell division process; the cells are reduced; and the electron transfer rate of shewanella is improved. The invention provides a new method for transforming electroactive microorganisms by using morphological engineering, enhancing the electron transfer capability of shewanella and improving the application capability of shewanella.

The invention relates to a combination therapy. In particular, a Cell Division Cycle 7 kinase inhibitor (CDC7i) used in a combination therapy with: i) a Cyclin Dependent Kinase 8 inhibitor (CDK8i), and / or ii) a Cyclin C inhibitor (CCNCi); for use in the treatment or prevention of cancer in a subject.

The invention relates to the technical field of fluorescence detection, in particular to a high-brightness fluorescent probe capable of rapidly and specifically staining cell nucleuses and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 2 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and the high-brightness fluorescent probe capable of rapidly and specifically staining the cell nucleuses is obtained. The green fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus within 1 minute is obtained. The method can be applied to rapid real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.