30 results about "Cell division cycle" patented technology

The invention relates to the field of medical food and discloses a tumor recovery type aquatic collagen peptide food. Every 100g of tumor recovery type aquatic collagen peptide food contains various nutritional ingredients, including 12.4-14.4g of tuna skin collagen peptide powder, 7-8g of shark skin collagen peptide powder, 3.5-4.5g of whey protein powder, 10-12g of MCT fat, 9-11g of LCT fat, 41-42g of carbohydrate, 2.4-2.6g of ganoderan, 2.4-2.6g of agaricus blazei murill polysaccharide, other vitamins and microelements. The tumor recovery type aquatic collagen peptide food is fit for tumor radiotherapy and chemotherapy patient, can intervene cell division cycle, is beneficial to inducing tumor cell apoptosis, can relieve the burden of visceral organ and enhance immunity of the organism, is fit for malnourished cancer patients including patients suffering from malignant diseases, anorexia, chewing dysphagia, and the like, can effectively relieve the side reaction appearing in the process of radiotherapy and chemotherapy and can effectively relieve the emesis symptom and reduce the tumor metastasis.

The invention discloses repair-type freeze-dried floccule. The repair-type freeze-dried floccule comprises the following raw materials in percentage by mass: 0.5-1% of sodium hyaluronate, 5-10% of collagen, 80-90% of water, 1-3% of a dendrobium officinale extract and 2-5% of oligopeptide-3. The repair type freeze-dried floccule can reconstruct a skin barrier, can quickly reach dermal-layer cells after being smeared on a skin, can be absorbed by a body even without being digested, and has super-strong cell regeneration and repair capability. Aged and atrophied cells are quickly replaced, the cell division period is prolonged, and linear arrangement of collagen and elastic fibers is promoted, so that the skin is intensively repaired, and the skin is restored to be young.

The invention discloses a brown planthopper N1cdc2 gene as well as an encoding product and an application of the brown planthopper N1cdc2 gene, and belongs to the technical field of biology. On the one hand, the invention discloses a brown planthopper N1cdc2 gene sequence and an encoding protein amino acid sequence of the brown planthopper N1cdc2 gene, and on the other hand, the invention also discloses an application of the brown planthopper N1cdc2 gene in control of the survival rate and the reproductive capacity of brown planthopper. Research is carried out mainly aiming at a cell division cycle gene N1cdc2 which is related to brown planthopper reproduction, the expression pattern and related function of the gene in brown planthopper reproduction expression are ascertained, the potential of the brown planthopper N1cdc2 gene as a new target for preventing an injurious insect is defined, and a foundation is established for development of an N1cdc2-based pest control new strategy.

The present invention relates to novel substituted [5,6]cyclic-4(3H)-pyrimidinone compounds of formula (I) and their preparation methods. (I) In particular, the present invention relates to novel substituted [5,6]cyclic-4(3H)- pyrimidinone compounds useful as inhibitors of protein kinases, specifically CDC7 (cell division cycle 7) inhibitors.

The invention relates to modulating Cdc14B levels (cell division cycle 14 homolog B) and/or Cdh1 (Fzr1 protein, CDC20-like 1b, or fizzy-related protein) levels to sensitize cells to DNA damage by increasing the abundance of Plk1 (polo-like kinase 1) in a target cell. In certain embodiments, the invention relates to modulating Plk1 levels, and in particular to increasing Plk1 levels, to sensitize target cells such as cancer cells to cell death or apoptosis. In certain embodiments, the invention relates to inhibitors of Cdc14B and Cdh1 that sensitize tumor cells to chemotherapy or radiation induced cell death or apoptosis. In addition to applications relating to cancer therapies and diagnostics, the Plk1 modulators and assays will be employed for identifying novel drugs or drug candidates useful for various proliferative and/or differentiative disorders such as major opportunistic infections, immune disorders, cardiovascular diseases and inflammatory disorders.

The invention relates to application of a micromolecule interference RNA to the preparation of medicines capable of regulating and controlling the tumor cell division cycle. The micromolecule interference RNA can submerge the survivin gene expression, regulate and control the cell cycles of the lung cancer, the gastric cancer, the hepatic cancer, the breast cancer and the cervical carcinoma with high efficiency, and remarkably inhibit the division of the cells of the lung cancer, the gastric cancer, the hepatic cancer, the breast cancer and the cervical carcinoma. The micromolecule interference RNA can be used for preparing the medicines capable of inhibiting tumor cell division.

The invention relates to the field of biological medicine, in particular to a use of a survivin gene targeted siRNA in preparing medicine capable of regulating and controlling tumour cell division cycle. Experiments indicate that the survivin gene targeted siRNA can change the cell division cycle of lung cancer cell, gastric cancer cell, liver cancer cell, breast cancer cell and leukemia cell, and obviously restrain the cell division of the lung cancer cell, gastric cancer cell, liver cancer cell, breast cancer cell and leukemia cell. The survivin gene targeted siRNA can be used for preparing the medicine capable of restraining the cell division of the tumour cell.

The present invention relates to uracil glycosidase process for detecting cell division cycle protein gene 6 in tumor. In the process of DNA extraction, RNA extraction, protein extraction, immunohistochemistry, reverse transcription (RT), PCR, Northern hybridizing, in-situ hybridizing and capillary electrophoresis, uracil glycosidase process is adopted for detecting cell division cycle protein gene 6 in tumor. The process has dU to replace dT in synthesizing the first pair of reactants, uracil glycosidase treatment before the second amplification to eliminate the residual pollutant of PCR product, and dUTP to replace dTTP in the second amplification for mixing great amount of dU into the product. The process can eliminate all pollutants of different sources.

The invention discloses a serum-free culture medium for induced pluripotent stem cells. The serum-free culture medium comprises a basic culture medium and the following components added into the basic culture medium: insulin, dichlorophenazine, heparin sulfate glycoprotein, furanodiene, D-menthone, kavalactone, IFN-gamma, EGF and zinc citrate. The dichlorophenazine, the heparin sulfate glycoprotein and the kavalactone which are added into the culture medium have a synergistic effect to regulate and control the mitosis cycle of the induced pluripotent stem cells, accelerate cell metabolism, shorten the cell division cycle and enable the cells to maintain a relatively high proliferation speed. Furandiene and D-menthone are helpful for the induced pluripotent stem cells to maintain proliferation activity and a relatively good growth state, and play a role in ensuring the stability of cell proliferation. IFN-gamma, EGF and zinc citrate provide nutrition supply for cell proliferation, and provide energy for cell proliferation together with the basic culture medium. The invention further provides a preparation method of the serum-free culture medium, the process is simple and easy to operate, and convenience is provided for commercialization of products.

The invention relates to a bathing box capable of prolonging life. The bathing box combines a physical mode, a chemical function and psychological induction together. In the aspect of the physical mode, the bathing box can perform mechanical sealing, heating, skin expanding through vacuum and free radial suction through negative pressure. In the aspect of the chemical function, Chinese medicinal herb bathing agents having the function of achieving composite displacement with the free radial (heavy metal toxin) are utilized. In the aspect of the psychological induction, popular science films and light music of the bathing box capable of prolonging the life are played. When the bathing box capable of prolonging the life is used, the popular science films and the light music can stimulate the brain and activate the skin so that under the vacuum state, skin cell sizes are enlarged, and capillaries are expanded. In addition, active ingredients of Chinese medicinal herbs enter the skin to react with the free radial containing heavy metal, and the free radial can be effectively separated from cells and is removed out of the skin, so that activity of the skin is recovered, immunity is improved, a cell division cycle and cell life are prolonged, and the purpose of rejuvenation and longevity of people is achieved.

The invention discloses a demethylation-based vector for promoting self-renewal and proliferation of germline stem cells and application thereof. The vector is coated in slow virus, and carries mTet1 gene. The vector is a slow virus vector, and the nucleotide sequence of the mTet1 gene is disclosed as SEQ.ID.NO.1. After the vector disclosed by the invention is exprssed through the slow virus, the mTet1 gene is integrated to the genome of the milk goat male germline stem cells. When the stable positive cells are cultured, the cells grow in a swirl cluster mode and have favorable cytomorphology; the stream-mode analysis on the cell division cycle detects that the quantity of S-stage cells is increased, which indicates that the proliferation capacity is enhanced; and the transcription level detects that the expression of the male germline stem cell related gene and self-renewal related gene is increased, and the expression of the differentiation related gene is decreased.

The present invention relates to compounds of formula I as defined herein, and salts and solvates thereof, that function as inhibitors of cell division cycle 7 (Cdc7) kinase enzyme activity. The present invention also relates to pharmaceutical compositions comprising them, and to their use in the treatment of proliferative disorders, such as cancer, as well as other diseases or conditions in which Cdc7 kinase activity is implicated.

The present invention relates to an antibody which specifically binds to TMAP (tumor associated microtubule associated protein)/CKAP2 (cytoskeleton associated protein 2) or a fragment thereof, and a method for identifying the presence or absence of mitosis and a method for diagnosing cancer prognosis using the same. More specifically, the present invention relates to a composition for diagnosing cancer prognosis comprising an anti-TMAP/CKAP2 antibody or an antigen-binding site thereof, a method for detecting TMAP/CKAP2 using the composition, an anti-TMAP/CKAP2 antibody for diagnosing cancer prognosis, a method for providing information for diagnosing cancer prognosis using the composition, a method for screening a cancer therapeutic agent comprising the step of determining changes in the level of TMAP/CKAP2 antigen-antibody reaction by the treatment of a candidate substance, and a composition for determining cell-division cycles using the composition.

The invention discloses novel purine analogue tetrazole (1, 5-a) pyrimidine-5, 7-diol (compound I) and a synthetic route thereof. Pharmacological experiments show that the compound I is inhibitory for cell division cycle phosphoesterase CDC25. The compound I can be made into anti-metabolites high in bioactivity, fine in selectivity and low in toxicity, and is widely applicable to the clinic.

Various compounds and pharmaceutically acceptable salts thereof are provided capable of binding cyclin-dependent kinase inhibitor 1B. The compounds can have a structure according to Formula I or Formula II as detailed herein. The compounds can include SJ747, SJ749, SJ755, SJ757. Pharmaceutical formulations containing the compounds or pharmaceutically acceptable salts are also provided along with methods of use thereof. The formulations and methods can be useful for treating cancer. In some aspects, the cancer is associated with a mislocalization of the intrinsically disordered protein p27. In some aspects, the cancer is resistant to an anticancer therapy. The pharmaceutical formulation can therefore include a second active agent and/or can be given in combination with a second active agent such as a cancer therapeutic. In various aspects, methods of promoting reentry into the cell division cycle in a subject in need thereof using compounds and formulations described herein are also provided.

Provided is a rabbit monoclonal antibody to acetylated mouse BubR1 and a preparation method therefor and, more particularly, provides the use of the monoclonal antibody in a method for measuring the activity of cell division checkpoints on the basis of a degree of acetylation of BubR1, a method for detecting a tumor disease on the basis of aberrant cell division, a method for diagnosing cancer, a method for screening anti-cancer agents, or a method for regulating a cell division cycle.

The invention discloses a novel purine analogue 7-amino-tetrazole[1,5-a]pyrimidine-5-alcohol(compound II) and its synthetic route. It shows through a pharmacological experiment that the compound II has an effect of inhibiting cell division cycle phosphoesterase CDC25. The compound II can be used as an antimetabolic medicine with high biological activity, good selectivity and little toxicity, and can be widely used in clinic.