6 results about "Cell division cycle" patented technology

The invention relates to the technical field of biology, in particular to desmosome-XVII type collagen fusion protein as well as a preparation method and application thereof. The invention provides a recombinant XVII type collagen fusion protein which comprises at least one XVII type collagen functional domain fragment and at least one functional domain fragment of desmosome core glycoprotein, the recombinant human X VII type collagen fusion protein provided by the invention has better cell adhesion activity and cell proliferation promoting activity. The recombinant human XVII type collagen fusion desmosis protein can significantly improve the adhesion between cells in a cell culture environment, and compared with commercial human XVII type collagen, the recombinant human XVII type collagen fusion desmosis protein can improve the effect by several times or more. In the aspect of promoting cell proliferation, the cell division cycle can be effectively shortened, so that the number of cells is rapidly increased within the same time, and the XVII type collagen is remarkably superior to commercial human XVII type collagen, and has wider application potential in the fields of skin care products, cosmetics, medicines, biomedical materials and the like.

The present application relates to substituted pyrrole carboxamide derivatives of Formula (I) that modulate the activity of the cell division cycle 7-related kinase (Cdc7). Accordingly, the compounds of the present application are useful in the treatment of diseases associated with dysregulated kinase activity, such as cancer, cell proliferative disorders, viral infections, immune disorders, neurodegenerative disorders, cardiovascular diseases, and bone-related diseases. The present application also provides methods for making these compounds, pharmaceutical compositions comprising these compounds, and their medical uses.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a compound capable of combining with cell division cycle 20 (Cdc20) and a composition thereof, and particularly discloses a compound capable of combining with cell division cycle 20 (Cdc20), wherein the compound is shown as (I); the compound can further become a targeted protein degradation agent and a pharmaceutical composition, and is used for treating or preventing diseases caused by promoting abnormal cell division after Cdc20 activates an APC complex, and the compound has high Cdc20 combining ability.

The invention relates to the technical field of fluorescence detection, in particular to a cell nucleus targeting yellow fluorescent probe and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted thio-piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 6 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and a target product is obtained; the yellow fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus is obtained. The method can be applied to real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.

The invention relates to a combination therapy. In particular, a Cell Division Cycle 7 kinase inhibitor (CDC7i) used in a combination therapy with: i) a Cyclin Dependent Kinase 8 inhibitor (CDK8i), and / or ii) a Cyclin C inhibitor (CCNCi); for use in the treatment or prevention of cancer in a subject.

The invention relates to the technical field of fluorescence detection, in particular to a high-brightness fluorescent probe capable of rapidly and specifically staining cell nucleuses and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 2 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and the high-brightness fluorescent probe capable of rapidly and specifically staining the cell nucleuses is obtained. The green fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus within 1 minute is obtained. The method can be applied to rapid real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.