Process of detecting cell splitting periodic protein gene 6 in tumour using heat starting polyases chain reaction
A cell division and cycle protein technology, applied in the field of detection of cell division cycle protein gene 6 in tumors, can solve the problems of cumbersome methods and easy pollution, and achieve the effects of ensuring reliability, preventing infection, and improving detection sensitivity and specificity
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[0011] 1. Randomly select cases
[0012] 30 cases of bladder cancer were diagnosed, including 6 cases of initial onset and 24 cases of recurrence; 26 cases of men aged 36 to 84; 4 cases of women aged 60 to 73.
[0013] 25 cases of other urinary system diseases were diagnosed, including 18 cases of males aged 28-77; 7 cases of females aged 27-69; causes: 4 cases of bladder stones; 4 cases of kidney stones; 10 cases of urinary tract infection; 2 cases of kidney cancer; epididymitis 1 case; 2 cases of benign prostatic hyperplasia; 2 cases of urethral stricture. 5 male healthy volunteers aged 29-31.
[0014] 2. Preparation of Urinary Exfoliated Cells
[0015] Take 150-200ml of random urine after the first urine in the morning, divide it into several treated glass centrifuge tubes, centrifuge at 4°C and 380g for 10 minutes, discard the supernatant, collect the precipitate in a centrifuge tube, and wash with normal saline The pellet was washed twice, and the pellet was divided in...
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