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Neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF (7,7''-Dimethoxyagastisflavone) and application of neoplasm metastasis suppressing pharmaceutical composition

A pharmaceutical composition and tumor metastasis technology, applied in the direction of drug combination, antineoplastic drugs, medical preparations containing active ingredients, etc., can solve the problems of unknown mechanism and application, failure to make further breakthroughs, and few studies on DMGF mechanism and efficacy , to achieve the effect of inhibiting tumor metastasis

Inactive Publication Date: 2015-11-25
SPRING FOUND OF NCTU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the current research on bissulfone compounds, it is known that they have a wide range of biological activities, such as antifungal, anti-inflammatory, antiviral, antioxidative, antitumor and other activities, however, different bissulfone compounds have different Biological activity, even though it also has anti-cancer potential, its mechanism and application are still largely unknown
[0004] Among the disulfonone compounds, a special class was also found, called DMGF for short (ie 7,7''-Dimethoxyagastisflavone), but there are quite few studies on the mechanism and efficacy of DMGF at present, and only found that it has the ability to inhibit yellow yeast Mold (Aspergillus flavus) secretion of aflatoxin (aflatoxin) efficacy, but follow-up studies have failed to make further breakthroughs

Method used

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  • Neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF (7,7''-Dimethoxyagastisflavone) and application of neoplasm metastasis suppressing pharmaceutical composition
  • Neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF (7,7''-Dimethoxyagastisflavone) and application of neoplasm metastasis suppressing pharmaceutical composition
  • Neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF (7,7''-Dimethoxyagastisflavone) and application of neoplasm metastasis suppressing pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental Example 1: Preparation of DMGF

[0032] The DMGF used in the present invention is an extract containing DMGF obtained by high performance liquid chromatography (High Performance Liquid Chromatography, HPLC), and the purity of the DMGF is above 98%. In this experimental example, DMGF was extracted in a Waters high-performance liquid chromatography system (Waters2796Bio-separationModule), wherein the column used in this experimental example is the column of the system, which is C18 with a particle size of 5mm TM Symmetry column (250mmx4.6mm), and by means of 30% methanol aqueous solution to wash the column at a speed of 0.5mL / min, and take the eluate that has a significant change in the absorbance at 254nm, which is Contains DMGF extract. Next, weigh about 5 mg of the aforementioned DMGF-containing extract at room temperature, and use this as a unit dose. Next, it is packaged in the form of a powder or cachet and stored at room temperature.

experiment example 2

[0033] Experimental example 2: DMGF has the effect of inhibiting the ability of tumor cells to degrade the extracellular matrix and inhibiting tumor metastasis

[0034] Using tissue culture techniques, mouse melanoma was cultured in cell culture medium DMEM (Gibco / Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco / Invitrogen, Carlsbad, CA, USA) and 1% antibiotics (mousemelanomacells) cell line B16F10 to an appropriate number, wherein the antibiotics are preferably penicillin / streptomycin / amphotericin. Next, take the containing quantity 3×10 4 The suspension of B16F10 cells was seeded in the upper chamber of two transwell culture dishes, one of which was added with DMGF at a final concentration of 1 μg / ml; the other was not added with DMGF as a control Group. In addition, cell culture medium DMEM was added to the upper and lower chambers of the permeable culture dish, and fetal bovine serum (FBS) was additionally added to the lower chamber. Then, put it ...

experiment example 3

[0038] Experimental Example 3: DMGF can inhibit the expression of matrix metalloproteinase-2 and inhibit the ability of tumor cells to degrade extracellular matrix

[0039] B16F10 cells were cultured by tissue culture technology, and the culture conditions were the same as those in Experimental Example 2. Take the containing quantity 1×10 6 The suspension of B16F10 cells was inoculated in at least three culture dishes, and two culture dishes were selected, and after adding the disulfonone compound DMGF with a final concentration of 1 μg / ml to the suspension of B16F10 cells, they were placed back in a 37°C Continue to cultivate in the incubator. After culturing for 12 and 24 hours respectively, the culture medium and the added compounds in the culture dish were sucked off, washed with phosphate buffered saline (PBS), and collected with trypsin-EDTA. B16F10 cells. And one of the dishes was not added with DMGF, and B16F10 cells were harvested according to the aforementioned met...

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Abstract

The invention discloses a neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF and an application of the neoplasm metastasis suppressing pharmaceutical composition. The neoplasm metastasis suppressing pharmaceutical composition comprises an effective dose of DMGF in respect of treatment and an acceptable carrier in respect of medicine. The invention further discloses the application of the neoplasm metastasis suppressing pharmaceutical composition prepared by DMGF. Neoplasm metastasis can be suppressed effectively by the neoplasm metastasis suppressing pharmaceutical composition and the application thereof.

Description

technical field [0001] The invention relates to a pharmaceutical composition for inhibiting tumor metastasis and the use of materials for preparing the pharmaceutical composition. Background technique [0002] Polyphenolic compounds (Polyphenolic compounds) are commonly found in the ingredients of general daily diet and herbal medicines, such as vegetables, fruits, beans, red wine, tea and ginkgo, etc., all contain different polyphenolic compounds. Different polyphenolic compounds can produce different biological activities on organisms, such as anti-inflammatory, anti-cancer or anti-aging effects. Among them, Paclitaxel (trade name: Taxol), which is extracted from the bark of Pacific yew (Taxus brevifolia Nutt.), has been successfully proven to have the potential to treat cancer, and has attracted the attention of the medical community. Therefore, the physiological activity of other components extracted from yew is very worthy of research and has the value of development. ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00A61P35/04A61P29/00
Inventor 廖光文林静敏林于铃
Owner SPRING FOUND OF NCTU
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