Method for large scale preparing Gliocladium chlamydospore

Abstract

The invention provides a method for preparing a large quantity of chlamydospore of Gliocladium virens, which comprises a culture medium formula and culture technology. A liquid medium comprises the following compositions: 20 to 25 grams per liter of glucose, 1 to 3 grams per liter of bean cake powder, 1 to 3 grams per liter of urea, 0.005 to 0.05 gram per liter of ferrous sulfate, 0.75 to 1.5 grams per liter of dipotassium hydrogen phosphate and distilled water. The liquid medium is used for fermentation culture of Gliocladium virens which have superparasitic function on various plant pathogenic fungi (such as sclerotinia, gray mold, banded sclerotial blight and pine root fungi). The culture time is calculated beginning from inoculation, and the chlamydospore of the Gliocladium virens begins to be greatly generated after 36 to 48 hours and is respectively and independently separated. The invention aims to provide the culture medium formula with lower price and rich sources of raw materials, wherein a large quantity of the chlamydospore of the Gliocladium virens can be prepared by utilization of the culture medium formula; and the repeatability is good and the cost is greatly reduced under the condition of liquid fermentation culture, so as to guarantee the biological activity and the prevention and treatment effect of the spores in production and application.

Description

Technical field: [0001] The invention relates to the technical field of microbes, in particular to a method for preparing a large amount of Gliocladium chlamydospores, including medium formula and culture technology. Background technique: [0002] In the biopesticides used to control plant diseases, microbial fungal preparations play an important role. Among them, Gliocladium spp. is considered to be one of the most promising plant disease biocontrol factors among the antagonistic microorganisms discovered so far, and can be used to control various plant diseases. [0003] Gliocladium is a soil habituating fungus that widely exists in various soil ecological environments. Common Gliocladium species include G. roseum, G. catenulatum and G. virens, etc., which have shown good control effects on plant diseases. To sum up, Gliocladium has great biocontrol potential due to its excellent characteristics as follows: ① It has a wide range of environmental adaptability, can use var...

AI Technical Summary

Problems solved by technology

The hyphae of Gliocladium are prone to dehydration and cause death under natural conditions, and although the conidia produce a large amount of spores, their survival period is also short, and usually 70% of the spores lose their vitality after a one-year storage period; Apparently, the two types of bacteria, conidia and mycelia, are difficult to meet the requirements of Gliocladium as a biopesticide preparation