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Method for extracting lycorine from Lycoris radiata

A technology of lycorine and lycorine, which is applied in the field of extraction of traditional Chinese medicines, can solve the problem of low extraction rate of lycorine, and achieve the effects of avoiding impurity dissolution, saving energy and improving content.

Active Publication Date: 2011-08-31
HUABAO FLAVOURS & FRAGRANCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The total alkaloids in Lycoris are often extracted with water or acid water-organic solvent or alcohol-acid water-organic solvent, such as Chinese patents and patent applications 200310102276.2, 200310108800.7, 200410022848.0, 200610051215.1, 200610110225.8, 200710053207.5, but these traditional methods Alkaline extraction rate is generally not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the present invention extracts the method for lycorine from lycoris bulb

[0051] Dry 5Kg of Lycoris bulbs at a temperature of 60°C for 50 minutes, then use a pulverizer sold by Shuanglong Group under the trade name SF series universal pulverizer to crush them into 30 meshes, and then add water to soak for 0.5 hours. The amount of water used is 15 times of weight, with 0.5% by weight of HCl solution, its lycoris soaking solution pH is adjusted to 4, then add the compound enzyme that is made up of 0.3% cellulase and 0.1% pectinase in terms of lycoris gross weight, then Put it into the mixing batching tank under the brand name of Wuxi Top Mixing Equipment Co., Ltd., heat it at a stirring speed of 180 rpm and a heating rate of 8 °C / min. When the temperature is raised to 50 °C, keep the temperature for constant temperature enzymolysis for 2 hours. Use the filter sold by Jiangsu Juneng Machinery Co., Ltd. under the trade name NYB type high-efficiency plate-type...

Embodiment 2

[0055] Embodiment 2: The present invention extracts the method for lycorine from lycoris bulb

[0056] Dry 5Kg of Lycoris bulbs at a temperature of 70°C for 40 minutes, then use a pulverizer sold by Shuanglong Group under the trade name SF series universal pulverizer to crush them into 30 meshes, and then add water to soak for 0.5 hours. The amount of water used is 20 times of weight, with 0.5% by weight of HCl solution, its Lycoris soaking liquid pH is adjusted to 4.5, then add the compound enzyme that is made up of 0.2% cellulase and 0.2% pectinase in terms of Lycoris gross weight, then Put it into the batching tank sold by Wenzhou Shenhua Light Industry Machinery Co., Ltd. under the trade name of magnetic stirring batching tank, and heat it at a heating rate of 10 °C / min at a stirring speed of 200 rpm. Enzymatic hydrolysis at constant temperature for 2.5 hours, using Yixing Changhua Filtration Equipment Co., Ltd. to filter and separate the residue of Lycoris enzymolysis wit...

Embodiment 3

[0060] Embodiment 3: Using single enzyme to extract lycorine from Lycoris bulbs for comparative implementation

[0061] Dry 5Kg of Lycoris bulbs at a temperature of 70°C for 40 minutes, then use a pulverizer sold by Shuanglong Group under the trade name SF series universal pulverizer to crush them into 30 meshes, and then add water to soak for 0.5 hours. The amount of water used is 20 times of weight, with 0.5% by weight of HCl solution, the pH of its Lycoris soaking solution is adjusted to 4.5, then add 0.2% cellulase based on the total weight of Lycoris, then put it into Wenzhou Shenhua Light Industry Machinery Co., Ltd. In the batching tank sold by the famous magnetic stirring batching tank, heat at a stirring speed of 200 rpm and a heating rate of 10°C / min. When the temperature rises to 55°C, keep the temperature for constant temperature enzymolysis for 2.5 hours, and use Yixing City Changhua to filter Apparatus Co., Ltd. obtains Lycoris enzymolysis residue by filtering an...

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PUM

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Abstract

The invention discloses an extraction and separation method for lycorine in Lycoris radiata. Lycoris radiata bulbs are taken as a raw material, and the lycorine is extracted by adopting complex enzyme pretreatment, dilute hydrochloric acid leaching, ultrasonic-assisted extraction, and chloroform extraction methods. An adopted novel process effectively combines an ultrasonic extraction method witha composite enzymatic hydrolysis method, which shortens extraction time, and also greatly improves the extraction rate of the lycorine; and the extraction rate reaches 0.97 percent, and is higher than that of the prior extraction process. The method has the advantages of mild reaction conditions, simple needed equipment, concise flow, and reasonable and feasible process.

Description

【Technical field】 [0001] The invention relates to a method for extracting traditional Chinese medicine. In particular the present invention relates to a method for extracting lycorine from Lycoris. 【Background technique】 [0002] Lycoris is a monocot class Amaryllidaceae (Amaayllidaceae) plant, which is a perennial herb. According to incomplete statistics, there are about 20 kinds of them all over the world, all of which are beautiful flowers, mainly distributed in China and Japan. There are many biologically active components in Lycoris, especially alkaloids. After research, the bulbs of Lycoris plants contain more than ten kinds of alkaloids, such as lycorine, lycorine, lycorine, and pseudolycorine. With the continuous research on the medicinal use of Lycoris alkaloids in recent years, many new therapeutic effects have been discovered. These alkaloids not only have anti-inflammatory, antibacterial and anti-cancer effects, but also inhibit cholinesterase and stimulate n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18
Inventor 谷向春罗昌荣
Owner HUABAO FLAVOURS & FRAGRANCES CO LTD