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Antineoplastic novel compound in immature exocarpof Juglans mandshurica Maxim, method for determining content thereof and use

A method of determination and compound technology, which is applied in the fields of antineoplastic drugs, medical preparations containing active ingredients, organic chemistry, etc., can solve the problem of lagging development of Beiqinglongyi medicinal resources, lack of Beiqinglongyi, and inability to control medicinal materials more effectively and the quality of preparations to achieve excellent anticancer activity and prolong survival time

Inactive Publication Date: 2009-07-01
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the basic research on its pharmacodynamic substances has not been carried out in depth, and the literature reports mostly stay on the pharmacological effects of its crude extract and lack the antitumor effect of its active ingredients (Ji Yubin, Ma Hongtu, Yang Bo, Ji Chenfeng. Bei Qinglongyi Research on the anti-tumor effect of different extraction parts. Chinese Herbal Medicine, 2004, 35(10): 1145-1147), which led to a serious lag in the development of Beiqinglongyi, a medicinal resource
[0004] There is no content determination method for the anti-tumor active ingredients of Beiqinglongyi in the existing methods, so the quality of medicinal materials and preparations cannot be controlled more effectively, and they do not have specificity, accuracy and reliability

Method used

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  • Antineoplastic novel compound in immature exocarpof Juglans mandshurica Maxim, method for determining content thereof and use
  • Antineoplastic novel compound in immature exocarpof Juglans mandshurica Maxim, method for determining content thereof and use
  • Antineoplastic novel compound in immature exocarpof Juglans mandshurica Maxim, method for determining content thereof and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The new anti-tumor compound in Beiqinglongyi is named Qinglongyisu A, and the structural formula of the new compound is:

[0023]

[0024] The method for assaying the content of the new anti-tumor compound in Beiqinglongyi adopts HPLC method to measure, take Qinglongyisu A reference substance, weigh it accurately, add methanol to make a solution containing 0.2mg per 1ml, as the reference substance solution, Take 1g of Beiqinglongyi powder passed through the No. 3 sieve, weigh it accurately, add 50% methanol and ultrasonically treat it for 30 minutes, let it cool down, weigh it again, make up the lost weight with 50% methanol, shake well, filter, and use as For the test solution, 10 μl each of the reference solution and the test solution were accurately drawn, injected into the liquid chromatograph, measured, and obtained.

[0025] The method for determining the content of the new anti-tumor compound in Beiqinglongyi is determined by HPLC method, which means that the ...

Embodiment 2

[0028] The content determination method of the new anti-tumor compound in Beiqinglongyi is determined by HPLC method, the chromatographic condition is to use octadecylsilane bonded silica gel as filler, and the mobile phase is a mixture of 0.25% glacial acetic acid (V / V) and methanol The volume ratio is 25:75, the detection wavelength is 350nm, the column temperature is 30°C, the number of theoretical plates is not less than 3000 based on the Qinglongyisu A peak, take an appropriate amount of Qinglongyisu A reference substance, weigh it accurately, and add methanol to make it Each 1ml of the solution containing 0.2mg, as the reference substance solution, take 1g of Beiqinglongyi powder passed through the No. Make up for the lost weight, shake well, filter, and as the test solution, accurately draw 10 μl each of the reference solution and the test solution, inject it into a liquid chromatograph, measure it, and get it.

Embodiment 3

[0030] Preparation of New Antitumor Compounds from Beiqinglongyi

[0031] Material source Beiqinglongyi is the immature fruit of Juglans Mandshurica MAXIM., which was collected in Wuchang County, Heilongjiang Province in mid-July.

[0032] Extraction and separation Soak fresh Beiqinglongyi with 75% ethanol for one week, extract three times in total, combine the extracts, concentrate under reduced pressure, and concentrate to a concentrated solution with a relative density of 1.10-1.14 (50°C), add 10 times the amount of water to the concentrated solution Disperse, centrifuge, take the supernatant, inject it into the treated Diaion HP-20 macroporous adsorption resin (the ratio of the concentrated solution to the resin is 1:50), flow rate 10ml / min, wash with 3 times the volume of the column bed respectively , wash with 30% ethanol of 5 times the volume of the column bed, collect the 30% ethanol eluate, evaporate the eluate to dryness under reduced pressure to obtain a residue, di...

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Abstract

The invention relates to a novel anti-tumor compound in north Juglans regia and a content assay method and application thereof. The prior method does not contain a method for measuring the content of anti-tumor effective ingredients of the north Juglans regia, so that the method can not effectively control the mass of medicines and preparation and does not have specificity, accuracy and reliability. The invention utilizes high-performance liquid chromatography to establish the content assay method which takes the novel anti-tumor compound in the north Juglans regia as a target. The structural formula of the novel compound is the right formula (I). The novel anti-tumor compound is measured by the HPLC method, and is applied in the field of medicine.

Description

Technical field: [0001] The invention relates to a new anti-tumor compound in Beiqinglongyi and its content determination method. Background technique: [0002] Cancer is a serious disease that threatens human life. Therefore, research on anticancer drugs has always been a hot spot in the world. Compared with chemical drugs, traditional Chinese medicine anti-tumor is unique in improving immunity and reducing mutagenic toxicity. Therefore, it is necessary to find anti-tumor traditional Chinese medicine with definite curative effect from the motherland's treasure house of medicine, clarify the material basis of its efficacy, and then develop a high-efficiency and low-toxicity drug. The new drug has become the focus of many years of research in the field of traditional Chinese medicine. [0003] Beiqinglongyi is the immature fruit of Juglans Mandshurica MAXIM., a plant of the family Juglans. It is abundant in the suburban counties of Northeast China. It is commonly used as a p...

Claims

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Application Information

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IPC IPC(8): C07C59/90A61K31/192A61K36/52A61P35/00G01N30/02
Inventor 刘丽娟李巍
Owner HEILONGJIANG UNIV
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