Method for extracting pinostrobin and beta-sitosterin from hickory nut epicarp

Abstract

The invention provides a method for extracting pinostrobin and beta-sitosterol from exocarp of kiskatom, which belongs to the technical field of pinostrobin and beta-sitosterol extracting methods. The method comprises the following steps: washing and crushing the expocarp of the kiskatom; and then diacolating and extracting the expocarp after the expocarp is swelled by ethanol, and concentrating the collected diacolation solution to prepare a concentrated solution; extracting the concentrated solution by an organic solvent to prepare extract dry powder; and separating the extract dry powder by silica gel column chromatography with the following steps: a) carrying out gradient elution on the extract dry powder by sherwood oil and a sherwood oil-ethyl acetate mixed solution with volume ratio of between 45:1 and 15:1 respectively, and combining two kinds of eluate; and b) carrying out the gradient elution on the combined eluate by the sherwood oil-ethyl acetate mixed solution with volume ratio of between 10:1 and 1:20, and concentrating and crystallizing the combined eluate in the reduced pressure to prepare pinostrobin coarse crystal and beta-sitosterol coarse crystal; and carrying out recrystallization treatment to prepare pinostrobin crystal and beta-sitosterol crystal. The method has the advantages of simple process and low manufacturing cost, and the contents of the prepared pinostrobin crystal and the beta-sitosterol crystal are more than 98 percent.

Description

technical field [0001] The invention belongs to the technical field of methods for extracting pinuncinol and beta-sitosterol, in particular to a method for extracting pinocytin and beta-sitosterol from pecan exocarp. Background technique [0002] Pecan Juglans mandshurica Maxim, is Juglandaceae, Pecan genus Carya plant, mainly produced in Zhejiang Lin'an, Chun'an and Anji and other places. When pecans are immature, there is a thick green exocarp on the outside of the stone. As the pecans mature and are picked and peeled, the color of the exocarp will gradually turn reddish brown. The peeled pecan exocarp has a very large amount, and the weight ratio of the fruit to the exocarp is almost 1:1, so it will cause great waste after discarding. According to literature reports, pecan leaves, bark and exocarp can be used as medicine. Pecan bark is bitter, acrid, and cold in nature, and can clear heat, dry dampness, clear intestines, and kill insects. In recent years, it has been u...

AI Technical Summary

Problems solved by technology

[0009] The existing domestic reports on the separation and purification of coniferin and β-sitosterol are limited to the systematic chemical composition research on plant raw materials, and the basic research reports on the isolation and identification of their chemical structures, but no extraction methods are given. The specific process parameters in the separation do not consider product purity, process methods and steps, production costs, environmental pollution, etc.

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