Method of measuring glycohemoglobin concentration and apparatus for concentration measurement

一种糖基血红蛋白、浓度测定的技术，应用在测量装置、颜色/光谱特性测量、仪器等方向，能够解决难测定等问题

Active Publication Date: 2009-07-15
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, under conditions such as an environment where the ambient temperature changes greatly, since the ratio of oxyhemoglobin and deoxyhemoglobin is different, it is difficult to perform accurate measurement when measuring them at the same wavelength.

Method used

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  • Method of measuring glycohemoglobin concentration and apparatus for concentration measurement
  • Method of measuring glycohemoglobin concentration and apparatus for concentration measurement
  • Method of measuring glycohemoglobin concentration and apparatus for concentration measurement

Examples

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Embodiment 1

[0108] In this example, in the case of measuring the glycohemoglobin concentration by changing the measurement wavelength, the influence of the ambient temperature on the measurement value was analyzed.

[0109] When the ambient temperature was 10°C, 20°C, and 30°C, the concentration of glycohemoglobin was measured using a glycohemoglobin measurement device (“ADAMS A1c HA-8160”: manufactured by ARKRAY, Inc. ) was measured using a photodiode array ("ultraviolet visible multi-wavelength detector MD-910"; manufactured by JASCO Corporation) as a photosensitive element. The concentration of glycohemoglobin is determined by the following method: within the wavelength range of 415-430nm, measure the total amount of hemoglobin and the amount of glycohemoglobin per 1 nm, respectively, and on this basis, calculate the concentration of glycohemoglobin in the above wavelength range The ratio of the cumulative value to the cumulative value of hemoglobin was taken as the glycohemoglobin con...

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PUM

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Abstract

When the concentration of glycated hemoglobin is measured, a plurality of wavelengths are selected as measurement wavelengths from the wavelength range of 400 to 450 nm. Preferably, by use of a liquid chromatography, at least the light of different peak wavelengths in the wavelength range of 415 to 430 nm are continuously or intermittently received to obtain a tree dimensional chromatogram having as variables the wavelength, the elution time and the amount of detection. The concentration of glycated hemoglobin is calculated based on this three dimensional chromatogram.

Description

technical field [0001] The present invention relates to a technique for measuring the concentration of glycohemoglobin contained in a sample such as blood. Background technique [0002] In the case of separating and analyzing body components using a body sample such as blood, a high-speed liquid chromatography device (HPLC device) using high-speed liquid chromatography (HPLC) is widely used (for example, refer to Patent Document 1). . [0003] Such as Figure 13 As shown, a general HPLC apparatus 9 is configured in such a manner that a sample including a body component is prepared in a sample preparation unit 90, and then the sample is introduced into an analysis column 91 to make the body The components are adsorbed in the packing of the analytical column 91 . When whole blood is used as the sample to measure glycohemoglobin, erythrocytes collected from the whole blood are hemolyzed to the analysis column 91, and the hemolyzed blood is diluted, and the biological sample i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27G01N30/74G01N30/88
CPCG01N21/31G01N2030/8822G01N30/8679G01N30/88G01N30/74
Inventor 杉山幸司酒井敏克
Owner ARKRAY INC
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