Microecological preparation and preparation method thereof
A technology of microecological preparations and preparations, which is applied in the field of animal microecological preparation products, waste water pollution control treatment and comprehensive utilization, which can solve the problems of blood and wastewater pollution, reduce harmful residues, prevent intestinal diseases, save pollution and control pollution The effect of fees
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manufacture Embodiment 1
[0019] Manufacture Example 1: Treat 100 kilograms of blood wastewater:
[0020] 1. Strain preparation: Saccharomyes cerevisiae Meyen exHansen CGMCC AS 2.121, L.acidophilus CGMCC 1.1854, and Bacillus cereus CGMCC AS 1.895 were selected as strains.
[0021] Preparation of seed liquid: culture medium: 75 grams of brown sugar, 15 grams of peptone, dilute to 3000 ml with blood wastewater, sterilize at 115°C for 20 minutes; insert the activated above-mentioned strains after cooling down, control the temperature at 34±1°C Cultivate for 36 hours to obtain a compound seed liquid;
[0022] 2. Fermentation: Put 100 kg of fresh blood wastewater into the fermentation device, sterilize, add 3 liters of compound seed liquid and stir evenly, control the temperature at 34±1°C, during the cultivation process, aerate and stir intermittently, cultivate for 48 hours, and test the pH3 .6. Fermentation is terminated; after inspection and packaging, it is the probiotics for animals.
manufacture Embodiment 2
[0023] Manufacture Example 2: Treat 100 kilograms of blood wastewater:
[0024] 1. Strain preparation: the strains were Saccharomyes cerevisiae Meyen exHansen CGMCC AS 2.610, Lactobacillus delbrueckii subspbulgaricus CGMCC AS 1.1482, and Bacillus subtilis CGMCCAS 1.140;
[0025] Preparation of seed liquid: culture medium: 100 grams of glucose, 100 grams of yeast extract, dilute to 10 liters with blood wastewater, sterilize at 121°C for 15 minutes; insert the activated above-mentioned strains after cooling down, control the temperature at 28± Cultivate at 1°C for 60 hours to obtain secondary compound seed liquid of yeast and lactic acid bacteria;
[0026] 2. Fermentation: Put 100 kg of blood wastewater into the fermentation device, add 2 liters of molasses, sterilize, add 10 liters of compound seed liquid, stir evenly, control the temperature at 28±1°C for 60 hours, test the pH3.4, and stop the fermentation. After inspection and packaging, it is the probiotics for animals.
manufacture Embodiment 3
[0027] Manufacture Example 3: Treat 100 kilograms of blood wastewater:
[0028] 1. Strain preparation: Saccharomyes cerevisiae Meyen exHansen CGMCC AS 2.121, Lactobacillus fermentum AS 1.122, and Bacillus licheniformis CGMCC AS 1.932 were selected as strains.
[0029] Seed preparation: medium: 10 grams of molasses, 6 grams of beef extract, dilute to 200 ml with blood wastewater, sterilize at 121°C for 15 minutes; insert the activated above-mentioned strains after cooling down, and control the temperature at 36±1°C for cultivation After 72 hours, the compound seed solution was obtained;
[0030] 2. Fermentation: put 100 kg of blood wastewater into the fermentation device, add 1 kg of glucose, sterilize, insert the above seed liquid, stir evenly, control the temperature at 36±1°C for cultivation, during the cultivation process, intermittently ventilate and stir, and cultivate for 72 The pH is detected at 3.5 hours, and the fermentation is terminated. After inspection and sub-p...
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