Immunodiagnosis kit for detecting IV-type dengue virus NS1 antigen

Abstract

The invention discloses an immunodiagnosis kit for detecting specific IV-type dengue virus antigen, comprising a microporous reaction plate coated with monoclonal antibody 2E8A5, sample treatment fluid, monoclonal antibody 7A6A1 combined with markers, positive control, negative control, wash concentrate, color developing solution and stop solution. The kit can detect IV-type dengue virus antigen specifically, does not cross-react with other three types of serotype dengue virus antigens and detects that the supernatant sensitivity of the IV-type dengue virus is 64 times of that of the commercial kit of the same type, namely, the Pan-E Dengue Early ELISA test kit, thus greatly improving the sensitivity of detection of clinical serum samples and simultaneously reducing omission rate in clinical applications.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a medical preparation, in particular to a kit for diagnosing type IV dengue virus. Background technique [0002] Dengue fever is a tropical infectious disease transmitted by Aedes aegypti and Aedes albopictus. According to the antigenicity of E protein, it is divided into four serotypes, namely type I, type II, type III and type IV (referred to as DV1, DV2, DV3 and DV4). Any type of dengue virus infection can cause a series of clinical symptoms, manifested as occult infection, fever, dengue fever, or more severe dengue hemorrhagic fever and dengue shock syndrome. In recent years, due to the development of international tourism and improper prevention and control measures for infectious diseases, dengue fever is widely prevalent in more than 100 countries and regions in the tropics and subtropics, especially in Southeast Asia. At present, about 2.5 billion people in the world are threate...

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Problems solved by technology

Furthermore, these two kits cannot distinguish between the four serotypes of dengue virus. Since the clinical symptoms and treatment options caused by different serotypes of dengue virus infection are different, clinicians must determine which serotype is different as soon as possible. dengue virus infection for rapid and effective treatment

Therefore, timely determination of the type of dengue virus infection is a prerequisite for reducing the mortality rate of dengue virus infection and an effective measure to control the spread of dengue virus. However, none of the commercial kits currently on the market can achieve this goal.

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