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Device and process for assays using binding members

A technology that combines components and covers components, applied in the field of analysis, and can solve problems such as difficulties

Active Publication Date: 2013-04-03
CLONDIAG GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, making conventional microfluidic devices suitable for coordinating various tasks can be difficult

Method used

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  • Device and process for assays using binding members
  • Device and process for assays using binding members
  • Device and process for assays using binding members

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0425] Example 1: Competitive Assay for Determination of Human Poliovirus 1 DNA

[0426] The schematic diagram of the performed competitive analysis is shown in Figure 22 middle. The DNA of human poliovirus 1 isolate TCDC01-861 (GenBank accession number AF538843) was cloned into an appropriate expression vector (pCR 2.1-TOPO , Clontech, Inc. Palo Alto, CA, USA), used as a DNA template (also referred to herein as "EV" (enterovirus) DNA).

[0427] contains 10 for each of the two 4 Samples of three DNA copies were analyzed in parallel: the first sample was PCR amplified using a Rotor-Gene 6000 real-time rotary PCR analyzer (Corbett Life Sciences, Sydney, Australia) according to the manufacturer's instructions.

[0428] The second sample also contains a Taqman The probe has the same nucleotide sequence, but contains a CY3 carbocyanine label (Invitrogen Corporation, Carlsbad, CA, USA) at its 3' end instead of a FAM / TAMRA-labeled reporter molecule, and is analyzed in a re...

Embodiment 2

[0445] Example 2: Array-Based Competitive Assay for Determination of HIV1 gag / env DNA

[0446] The schematic diagram of the competitive analysis performed is shown in Figure 24 In A. The DNA of the synthetic HIV1 gag / env fusion construct (EMBL Accession No. A06258) was cloned into the expression vector pCR 2.1-TOPO (Clontech, Inc. Palo Alto, CA, USA) EcoRI endonuclease restriction sites were used as DNA templates.

[0447] Additionally, the following PCR primers were used:

[0448] Forward PCR primers:

[0449] cdia: 5′-TGAAGGGTACTAGTAGTTCCTGCTATGTC-3′

[0450] (corresponding to nucleotides 214-232 of A06258)

[0451] Reverse PCR primers:

[0452] cdis: 5′-ATCAAGCAGCCATGCAAATGTT-3′

[0453] (corresponding to nucleotides 384-405 of A06258)

[0454] Thus, PCR resulted in the amplification of a 151 bp DNA fragment having the following sequence: 5'-ATC AAG CAG CCA TGC AAA TGT TAA AAG AGA CCA TCAATG AGG AAG CTG CAG AAT GGG ATA GAT TGC ATC CAG TCCATG GAG GGC CTA TTG C...

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Abstract

A device comprising a structure adapted for accomodating liquids, wherein the structure comprises a first binding member adapted for capturing target compounds and a second binding member adapted for capturing reporter compounds indicative of the presence and / or amount of the target compounds.

Description

[0001] priority statement [0002] This application claims priority to US Application No. 60 / 951,364, filed July 23, 2007, and US Application No. 60 / 856,782, filed November 6, 2006, each of which is incorporated by reference in its entirety. field of invention [0003] The present invention relates to methods of analysis, for example of polynucleotides. [0004] related application [0005] This application is related to the US continuation of the International Patent Application PCT / EP2005 / 004923 filed on May 6, 2005, which designates the United States and claims the German patent application DE 10 2004 022 263 filed on May 6, 2004 US Continuing Application No. US 11 / 593,021 entitled "Methods and Apparatus for Detecting Molecular Interactions" and filed November 6, 2006, each of which is hereby incorporated by reference in its entirety. Background of the invention [0006] The presence of a pathogen in a biological sample can be determined by analyzing the sample for poly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/00C12Q1/68B01L3/00
CPCB01J2219/00605B01L2300/0681B01L2400/0481B01J2219/00722B01L2300/0636B01J2219/00704B01L2400/0487B01J19/0046B01L9/527B01L7/52B01J2219/00695B01L3/502738B01L3/502761B01J2219/00659B01J2219/0072B01J2219/00527B01L2300/0816B01L3/502746B01J2219/00576C12Q1/6813B01L2200/10B01L3/502753B01L2200/0647B01L2400/0655C12Q1/6851C12Q2563/107C12Q2565/629C12Q2545/113C12Q1/6825C12Q1/6837C12Q1/701C12Q1/703C12Q2600/158
Inventor 卡特林·施泰因梅策尤金·埃曼特劳特托尔斯滕·舒尔茨托马斯·凯泽
Owner CLONDIAG GMBH