Sterilization method
A fungicide, weight technology, applied in the fields of botanical equipment and methods, biocides, sterilization/microdynamic water/sewage treatment, etc., can solve the problems of loss of microbicidal efficacy, instability, etc.
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Embodiment 1
[0023] Add 4,5-dichloro-n-octyl-3-isothiazolone (Dalian Xingyuan Fine Chemical Co., Ltd.), tributyl-tetradecyl phosphonium chloride (Huai'an) into a 100ml glass bottle equipped with a magnetic stirring rod. City Tianchi Chemical Co., Ltd.), dibutyl hydroxytoluene (Shanghai Beiji Biological Technology Co., Ltd.), dodecyl bis(aminoethyl) glycine (Shanxi Xinhe Taiming Chemical Co., Ltd.) and deionized water are mixed and stirred, Get sample 1#.
[0024] The composition of sample 1# is: 4,5-dichloro-n-octyl-3-isothiazolone 6 wt%, tributyl-tetradecyl phosphonium chloride 5 wt%, and dibutyl hydroxytoluene 1 wt% , Dodecylbis(aminoethyl)glycine 2% by weight, deionized water 86% by weight.
[0025] The following describes the static bactericidal effect of heterotrophic bacteria, sulfate-reducing bacteria and iron bacteria using the method of sample 1#.
[0026] (1) Take the strain to be tested and cultivate on the slant medium for 72 hours. The medium is prepared: 15 grams of agar, add appr...
Embodiment 2
[0036] Add 4,5-dichloro-n-octyl-3-isothiazolone, tributyl-tetradecylphosphonium chloride, dibutylhydroxytoluene, dodecane into a 100ml glass bottle equipped with a magnetic stirring rod Di(aminoethyl)glycine and deionized water were mixed and stirred to obtain sample 2#.
[0037] The composition of sample 2# is: 4,5-dichloro-n-octyl-3-isothiazolone 8% by weight, tributyl-tetradecylphosphonium chloride 8% by weight, and dibutylhydroxytoluene 3% by weight , 1% by weight of bis-octylaminoethylglycine, 80% by weight of deionized water.
[0038] Using the 2# sample method, the static sterilization effect of heterotrophic bacteria, the sterilization effect of sulfate reducing bacteria and iron bacteria are shown in Table 1, Table 2 and Table 3, respectively.
Embodiment 3
[0040] Add 4,5-dichloro-n-octyl-3-isothiazolone, tributyl-tetradecylphosphonium chloride, dibutylhydroxytoluene, dodecane into a 100ml glass bottle equipped with a magnetic stirring rod Di(aminoethyl)glycine and deionized water were mixed and stirred to obtain sample 3#.
[0041] The composition of sample 3# is: 4,5-dichloro-n-octyl-3-isothiazolone 4% by weight, tributyl-tetradecylphosphonium bromide 10% by weight, and dibutylhydroxytoluene 2% by weight , Bis-octylaminoethyl glycine 5% by weight, deionized water 79% by weight.
[0042] Using the method of 3# sample, the static sterilization effect of heterotrophic bacteria, the sterilization effect of sulfate reducing bacteria and iron bacteria are shown in Table 1, Table 2 and Table 3, respectively.
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