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Portable electrochemical biosensor for fast detecting organophosphorus pesticide residue

A pesticide residue and organophosphorus technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of insufficient design of detectors, manual recording of readings and calculations, and insufficient automation, achieving low-cost mass production and simple circuits , the effect of improving accuracy

Inactive Publication Date: 2009-11-25
左少华 +2
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Since there is only one working electrode on the sensor test strip, the working electrode is actually tested twice during the actual detection, and the enzyme layer may be damaged during the first test operation, resulting in deviations in the final results
The design of the detector is not simple enough, there are many switches and buttons, the operation process also needs to manually record the readings and calculations, and the degree of automation is not enough

Method used

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  • Portable electrochemical biosensor for fast detecting organophosphorus pesticide residue
  • Portable electrochemical biosensor for fast detecting organophosphorus pesticide residue

Examples

Experimental program
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Embodiment Construction

[0011] 1. Printing process of sensor electrode test strip:

[0012] (1) Use 0.5mm thick PET board as the substrate. Clean the substrate surface with water and acetone.

[0013] (2) Using a screen printing process, CH-10 conductive carbon paste (Jujo Co., Ltd., Japan) was printed on the substrate; thermal curing was performed in an oven at 120° C. for 30 minutes.

[0014] (3) Print a mixture of Prussian blue and CH-10 conductive carbon paste (1:4); heat curing in an oven at 120°C for 30 minutes.

[0015] (4) Print AC-3G (Jujo Co., Ltd., Japan) insulating ink; heat curing in an oven at 120°C for 5 minutes.

[0016] (5) Print the mixture of acetylcholinesterase and 5% chitosan solution; dry naturally.

[0017] (6) Process a sample pool with a 0.5mm thick PET plate, and stick it on the test strip by double-sided adhesive.

[0018] 2. The microcontroller of the portable testing instrument is C8051F040, which integrates analog / digital conversion and digital / analog conversion fun...

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Abstract

A portable electrochemical biosensor for fast detecting organophosphorus pesticide residue mainly comprises disposable sensor electrode reagent strips and a portable detecting instrument. The sensor electrode reagent strips are manufactured by a screen printing technique and each provided with two working electrodes and a shared counter electrode to be able to detect check samples and working samples at the same time and effectively reduce the result inaccuracy resulted from bad consistency of the manufacturing of the reagent strips; and the portable detecting instrument is designed on the basis of embedded system of a microcontroller, has two paths of sampling channels, a liquid crystal display and the alarm and reminding functions of a buzzer, and is powered by batteries. The portable electrochemical biosensor for fast detecting organophosphorus pesticide residue is based on the principle of electrochemical ferment sensor, and can fast detect the suppression ratio of the organophosphorus pesticide residue to acetylcholinesterase on spot, so as to evaluate the pesticide residue in the samples.

Description

technical field [0001] The invention relates to a portable electrochemical biosensor, in particular to a portable electrochemical biosensor for rapidly detecting organophosphorus pesticide residues. Background technique [0002] Organophosphorus and carbamate pesticides are a class of pesticides widely used in my country. Many of these pesticides are highly toxic. Once it enters the human body, it will irreversibly inhibit the activity of cholinesterase in the body, thereby affecting the normal function of the human central nervous system, and can lead to death in severe cases. In order to ensure the quality and safety of agricultural products, the state banned the production and distribution of five highly toxic organophosphorus pesticides including methamidophos in January 2008. On crops such as vegetables and fruits, relevant laws and regulations also restrict or prohibit the use of organophosphorus pesticides. However, illegal use and abuse of pesticides still exist. ...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/406G01N27/403
Inventor 左少华邬贤波左鹏
Owner 左少华
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