Method for extracting catechin

A technology of catechins and raw materials, which is applied in the field of extracting and separating compound catechins, to achieve the effect of large extraction amount, high extraction efficiency and wide range of biological activities

Inactive Publication Date: 2009-12-02
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The researches of the genus Miriam mainly focus on the indole alkaloids, and the pharmacological studies are quite clear, mainly focusing on anti-

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The extraction method of the catechin of the present embodiment comprises the following steps:

[0017] (1) After taking the dried cap stamen veneer (3kg) and pulverizing it, cold-soak 3 times at room temperature with 70% acetone aqueous solution, each time for 7 days, recover the reagent under reduced pressure to obtain the extract extract, and suspend the extract in water , sequentially extracted with petroleum ether, ethyl acetate and n-butanol;

[0018] (2) 35 g of ethyl acetate extract was mixed with 60-100 mesh silica gel according to the weight ratio of 1:30 and then loaded into the column. Petroleum ether and acetone were eluted at the volume ratio of 9:1. The solvent is recovered under pressure to obtain the crude product of catechin;

[0019] (3) Add an appropriate amount of acetone to the crude product, heat, then gradually add acetone until the crude product just dissolves; then add ether with 1 / 10 of the volume of acetone, let it stand, precipitate crystal...

Embodiment 2

[0021] The extraction method of the catechin of the present embodiment comprises the following steps:

[0022] (1) Take 1kg of the leaves of S. chinensis, dry and pulverize them, and cold-soak them with ethyl acetate solution at room temperature for 3 times, each time for 7 days, recover the reagent under reduced pressure to obtain the extract extract, and suspend the extract in water. , sequentially extracted with petroleum ether, ethyl acetate and n-butanol;

[0023] (2) 15g of ethyl acetate extract was mixed with 60-100 mesh silica gel according to the weight ratio of 1:30 and then loaded into the column. Petroleum ether and acetone were eluted at the volume ratio of 10:1. The solvent is recovered under pressure to obtain the crude product of catechin;

[0024] (3) Add an appropriate amount of acetone to the crude product, heat, and then gradually add acetone until the crude product just dissolves; then add ether with 1 / 9 of the volume of acetone, leave it to stand, precip...

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Abstract

The invention discloses a method for extracting catechin, comprising the following steps: (1), a raw material of rubiaceae mitragynaKorth plant cap core wood is subjected to cold soaking by using a solvent at room temperature after being dried and pulverized, an extract is obtained after the reagent is recovered by decompression, the extract is suspended in water, and sherwood oil, ethyl acetate and normal butanol are sequentially used for carrying out extraction; (2), an extract of the ethyl acetate is packed by silica gel of 60-100 meshes according to the weight ratio of 1 to 30 after sample stirring, the sherwood oil and acetone are eluted according to the volume ratio of 9:1-10:1, thin-layer detection is carried out, the similarities are combined, the solvent is recovered by decompression, and a crude product of catechin is obtained; and (3), the acetone is added into the crude product, the mixture is heated, the acetone is gradually added into the mixture until the crude product is dissolved, aether with the volume of one tenth to one ninth of the volume of the acetone is added into the mixture, and crystals are separated out by standing and are filtered to obtain a pure product of the catechin. The invention has simple extracting method, abundant sources of the raw materials and low cost, a catechin compound obtained by extracting has extensive bioactivity, a catechin monomer is obtained by extracting, the solvent used in the extracting process can be recycled, the extracting quantity is large and industrial production can be realized. By adopting the method, the extracting efficiency is high, and the yield reaches 50-90 percent.

Description

technical field [0001] The invention relates to a method for extracting catechins, in particular to a method for extracting and separating compound catechins from the medicinal plant Rubiaceae genus Milocarpus. Background technique [0002] There are many kinds of catechin compounds, among which flavan compounds such as catechin, epicatechin, and gallocatechin are represented, and they are widely distributed in plants, among which legumes (such as catechin, duck soap tree) , Rubiaceae (such as Uncaria catechu), Rosaceae (such as Potentilla, mountain cherry, Potentilla velvet, hawthorn), Polygonaceae (such as rhubarb, ginseng, and rhubarb), Camellia (such as camellia) , Aesculus (such as red horse chestnut, California horse chestnut) is more. Catechin has obvious biological activities such as scavenging free radicals in the body, anti-cancer, anti-inflammation, anti-mutation, anti-aging and improving liver function, and can be used for human health care and meat preservation...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61K36/74
Inventor 康文艺宋艳丽姬志强王金梅
Owner HENAN UNIVERSITY
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