Application of 2-methyl-5-isopropylphenol in Stellera chamaejasme L. in pesticides
A technology of isopropyl phenol and Ruixiang wolf poison is applied in the field of pesticides to achieve the effects of easy chemical synthesis and simple structure
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Embodiment 1
[0015] Example 1 The extraction of 2-methyl-5-isopropylphenol
[0016] (1) Wash the chamaejasma root, cut it into pieces, put it in a blast oven at 60°C for 2 days, grind it into powder with a grinder, and store it in the refrigerator for later use.
[0017] (2) Place the Rhizoma chamaejasma root powder prepared in step (1) in a Soxhlet extractor, add 8 times the weight of petroleum ether (60-90°C), extract at 70°C for 8 hours, filter under reduced pressure and Concentrate the filtrate to a semi-solid state to obtain a crude extract.
[0018] (3) Coarse division of large columns
[0019] Column diameter: 6cm; column height: 40cm; stationary phase is silica gel H (200-300 mesh); mobile phase is petroleum ether-ethyl acetate (volume ratio is 5:1 and 1:1); conventional wet method with petroleum ether Column packing. Take the crude extract as the separated sample, the sample volume is 10g each time, and the flow rate is 10ml min -1 , gradient elution (the volume ratio is about...
Embodiment 2
[0044] Example 2 Toxicity determination of 2-methyl-5-isopropylphenol to broad bean aphid
[0045] Taking the ultra-efficient insecticide imidacloprid as a contrast, carry out the indoor toxicity determination of the broad bean aphid to 2-methyl-5-isopropylphenol and imidacloprid according to the toxicity determination method described in Example 1, and then according to the probability value analysis method , using SPSS software to calculate LC 50 Value and confidence interval thereof, and find correlation coefficient and toxicity regression equation, the results are shown in Table 2 (the 2-methyl-5-isopropyl phenol used in this embodiment can be purchased from the market, and can also be obtained through embodiment 1 prepared by the method described).
[0046] Table 2 Toxic activity of 2-methyl-5-isopropylphenol on broad bean aphid
[0047]
[0048] The mortality rate values in Table 2 are the average values of 3 repeated determinations and corrected according to Ab...
Embodiment 3
[0051] Example 3 Toxicity determination of 2-methyl-5-isopropylphenol to armyworm
[0052] Taking beta-cypermethrin as a control, 2-methyl-5-isopropylphenol and beta-cypermethrin were dissolved in dimethyl sulfoxide and diluted with an aqueous solution of 0.1% Tween-80 by weight to form 5 different concentrations of solutions to be tested . Cut the corn leaves into 2×5cm sections, soak them in the solution to be tested for 5s, remove them, drain them, put them into a petri dish (6cm) with filter paper at the bottom, insert 10 third-instar larvae, and culture Dish is placed in the temperature and is 22~24 ℃, and relative humidity is 60%, and light time is 14:10h laboratory and continues to raise, record death situation after 24h, the test solution of each concentration repeats experiment 3 times (the present embodiment The 2-methyl-5-isopropylphenol used can be purchased from the market, or can be prepared by the method described in Example 1).
[0053] Correct the mortality ...
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