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Biological polypeptide medical device and manufacturing method thereof

A bio-peptide, medical device technology, applied in the direction of surface coating devices, stents, anodizing, etc., can solve the problems of unsafe, biological products falling off, falling off, etc., to avoid inflammation and side effects, to ensure effectiveness, The effect of specific capture

Active Publication Date: 2012-10-03
LEPU MEDICAL TECH (BEIJING) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the firmness between this layer and the stent body is not very good, and it is easy to fall off from the stent body during the pre-installation and expansion process of the stent, which will cause the biological products coated on the outside of the matrix layer to fall off, and the effective therapeutic dose cannot be achieved.
[0006] 2. Although these biological products can effectively capture cells containing specific markers, some of these specific markers are not unique to endothelial progenitor cells and vascular endothelial cells. For example, CD34+ cells may differentiate into vascular endothelial cells in vivo, and may Differentiate into other cells such as vascular smooth muscle cells, so CD34 antibody may promote the proliferation of smooth muscle cells while exerting the advantages of repairing endothelium, and may introduce unsafe factors while exerting advantages

Method used

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  • Biological polypeptide medical device and manufacturing method thereof
  • Biological polypeptide medical device and manufacturing method thereof
  • Biological polypeptide medical device and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation of embodiment 1 penicillamine cyclic peptide scaffold

[0059] The 316L stainless steel vascular stent was cut, slag-removed, and polished, placed in 75% medical alcohol, cleaned with ultrasonic waves at a frequency of 100khz for 10 minutes, set the temperature at 40°C, dried for 40 minutes, and then taken out.

[0060] Then the support is placed in 15% hydrochloric acid corrosion for 12 hours, the support body is connected to the positive pole of the pulse power supply as the anode, and the titanium metal sheet is connected to the negative pole of the pulse power supply as the cathode, and the support body and the cathode metal sheet are simultaneously placed in a concentration of 15% hydrochloric acid, the current is set to 1A, the frequency is 500 Hz, and the time is 15 minutes, holes are prepared on the surface of the 316L bare metal stent body. Such as figure 1 As shown, under the electron microscope scanning, it can be seen that there are obvious ...

Embodiment 2c

[0063] Example 2 Preparation of cyclo-RGD-SAA polypeptide scaffold

[0064] The 316L stainless steel vascular stent was cut, slag-removed, and polished, placed in 75% medical alcohol, cleaned with ultrasonic waves at a frequency of 100khz for 10 minutes, set the temperature at 40°C, dried for 40 minutes, and then taken out.

[0065] According to the set procedure, laser is used to drill holes on the surface of the bracket. The width of the hole on the outer surface of the bracket is 0.0003mm, and the depth is 0.0002mm.

[0066] Clean the above-mentioned treated stent with acetone solution with a concentration of 99.5%, and then ultrasonically clean it with distilled water at a frequency of 100khz for 10 minutes. Finally, place the cleaned stent in a dryer, set the temperature at 37°C, and take it out after drying for 30 minutes. Standby; use distilled water to prepare a hydrochloric acid solution with a concentration of 15%, soak the stent in the prepared solution, place it in...

Embodiment 3

[0068] Embodiment 3 Preparation of glycine-arginine-glycine-aspartic acid-serine-tyrosine scaffold

[0069] The 316L stainless steel vascular stent was cut, slag-removed, and polished, placed in 75% medical alcohol, cleaned with ultrasonic waves at a frequency of 100khz for 10 minutes, set the temperature at 40°C, dried for 40 minutes, and then taken out.

[0070] Glycine-arginine-glycine-aspartic acid-serine-tyrosine was prepared into a 5mg / ml aqueous solution, the above-mentioned 316L stainless steel vascular stent was placed on the place to be sprayed by ultrasonic spraying, and the nozzle and 316L stainless steel vascular stent Set up the electrostatic generator, the voltage of the electrostatic generator is 100V, the spraying flow rate is set to 1ml / min, and the spraying time of each circle is 1min. After a total of 3 spraying circles, take it out and store it at 4°C for a long time.

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Abstract

The invention relates to a novel biological polypeptide medical device and a manufacturing method thereof. The medical device adopts a principle of electrostatic adsorption and / or micropore physisorption and is manufactured by directly coating and / or immobilizing a biological antibody on the surface of the body of the device. In a manufacturing process of the medical device provided by the invention, the surface of the body of the device is not coated with substrate, so inflammation and other side effects caused by the substrate are avoided. In addition, the biological antibody on the surfaceof the body of the device has excellent biological activity, and can bear long-time washing by blood flow and other body fluids, ensure stable treatment effect and prevent restenosis and late thrombosis.

Description

technical field [0001] The invention relates to a medical device, in particular to a biological polypeptide medical device capable of reducing restenosis rate and a preparation method thereof. Background technique [0002] Since 1987, Sigwart et al. used intravascular metal stents for the first time in coronary arteries, which provided a good way for the treatment of vascular occlusion diseases. However, in-stent restenosis has always been the main reason affecting the efficacy of percutaneous coronary intervention (PCI). With the listing of Johnson & Johnson's Cypher rapamycin drug stent in 2004 and Boston Scientific's Taxus paclitaxel drug stent in 2005, drug-eluting stents have reduced the in-stent restenosis rate from 30% in the bare metal stent era to less than 10%. Therefore, the drug-eluting stent is considered to be the third milestone in the field of cardiac intervention after percutaneous coronary angioplasty and stent technology. [0003] In the first few years ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/10C25D11/02B05D7/24B05D1/18B05D1/28B05D1/02B05D1/04B05D3/00A61F2/82
Inventor 余占江韩雅玲邓捷张正才邱笑违陈永强冉玉凤张萌
Owner LEPU MEDICAL TECH (BEIJING) CO LTD
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