Bacillus subtilis oil suspending agent and preparation method thereof

A technology of Bacillus subtilis and oil suspension, which is applied in the field of oil suspension and its preparation, which can solve the problems of Bacillus subtilis loss, short storage time of water solution, increase of humidity in greenhouses, etc., and achieve high efficacy of pesticide application and increase of shelf life , the effect of increasing the survival time

Active Publication Date: 2009-12-30
海南利蒙特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the Bacillus subtilis produced at home and abroad are all wettable powders and water preparations. Because Bacillus subtilis is a living microorganism, the storage time of the water preparation is short, up to three months, which affects the use.
The wettable powder of Bacillus subtilis has a relatively long storage time, but the spore count drops more when the storage con...

Method used

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  • Bacillus subtilis oil suspending agent and preparation method thereof
  • Bacillus subtilis oil suspending agent and preparation method thereof
  • Bacillus subtilis oil suspending agent and preparation method thereof

Examples

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Embodiment 1

[0031] Embodiment 1: the preparation of bacillus subtilis oil suspension concentrate of the present invention

[0032] Weigh 2×10 11 200 grams of Bacillus subtilis parent powder of live spores / gram, 750 grams of methylated sunflower vegetable oil, 50 grams of OP-10 surfactants, mixed in a mixing kettle, and pulverized in a pump into a superfine pulverizing sand mill for 3 Hours, a stable oil suspension can be formed, the particle size is detected to be below 5 μm, and the concentration of Bacillus subtilis is 4×10 10 Viable spores / g.

Embodiment 2

[0033] Embodiment 2: the preparation of bacillus subtilis oil suspension concentrate of the present invention

[0034] Weigh 2×10 11 200 grams of Bacillus subtilis per gram of live spores, 600 grams of diluent (sunflower vegetable oil of methyl esterification), 200 grams of OP-10 surfactant, mix in a mixing kettle, and then pump into a superfine pulverizing sand mill Grinding in medium for 3 hours to form a stable oil suspension, the particle size is detected to be below 5 μm, and the concentration of Bacillus subtilis is 4×10 10 Viable spores / g.

Embodiment 3

[0035] Embodiment 3: the preparation of bacillus subtilis oil suspension concentrate of the present invention

[0036] Weigh 2×10 11 Live spores / gram Bacillus subtilis mother powder 10 grams, diluent (sunflower seed vegetable oil) 800 grams, SPAN-20 surfactant 190 grams, mix in the mixing kettle, then pump into the superfine pulverizing sand mill and pulverize 3 Hours, a stable oil suspension is formed, the particle size of the detected particles reaches below 5 μm, and the concentration of Bacillus subtilis is 2×10 9 Viable spores / g.

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Abstract

The invention provides a bacillus subtilis oil suspending agent, which comprises the following compositions in percentage by weight: 1 to 20 percent of 2*10<11> live gemmas/gram bacillus subtilis, 60 to 80 percent of diluent and 5 to 20 percent of surfactant. The invention also provides a preparation method of the bacillus subtilis oil suspending agent, which is to add bacillus subtilis yeast powder or spore powder into the diluent and the surfactant, evenly disperse the thallus, and crush to collid with grain diameter of below 5mu m to obtain the bacillus subtilis oil suspending agent. The bacillus subtilis oil suspending agent has the characteristics of high medicinal effect, low cost, and small environmental pollution and toxic hazard to operators, is a novel microbe fungicide, and has wide application prospect.

Description

technical field [0001] The invention relates to an oil suspension and a preparation method thereof, in particular to a bacillus subtilis oil suspension and a preparation method thereof. Background technique [0002] Bacillus subtilis, English name (Bacillus subtilis), is a kind of Bacillus. A single cell is 0.7-0.8×2.0-3.0 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, dirty white or yellowish. Bacillus subtilis can use protein, various sugars and starch to decompose tryptophan to form indole. Some strains are important producers of α-amylase and neutral protease; some strains have enzymes that strongly degrade nucleotides, so they are often used as parent strains of nucleoside-producing bacteria or to pr...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N63/02A01P3/00
Inventor 刘国忠张洁岩刘勇李新
Owner 海南利蒙特生物科技有限公司
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