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Method for preparing saponins compounds by fermenting and culturing gen-seng fruits

A technology of fermentation culture and American ginseng, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of simple extraction process, saving farmland, and no pesticide and heavy metal residues.

Inactive Publication Date: 2010-01-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the demand for saponin compounds in the market is growing rapidly, but the current production method cannot meet the demand of the market

Method used

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  • Method for preparing saponins compounds by fermenting and culturing gen-seng fruits

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Effect test

Embodiment Construction

[0024] Now take the laboratory culture as an example, and the non-limiting examples are described as follows:

[0025] 1. Preparation and sterilization of culture medium

[0026] Take fully mature Western fruits, crush them, remove the seeds, together with 650-700ml of pulp, peel and juice, 65-70g of glucose, 1-3g of ammonium sulfate, and then dilute to 1 liter with distilled water. Use citric acid to adjust the pH value to 3.2-3.5.

[0027] with sulfurous acid or SO 2 Sterilize. Dropping and stirring methods are used to make sulfurous acid or SO 2 After adding fruit juice, it is uniform and consistent to achieve a bactericidal effect.

[0028] 2. Seed cultivation

[0029] Inoculate the wine yeast into the culture medium and culture in dark at 27-28°C to induce and cultivate the mother species until the number of yeasts in the mother species reaches ≥10 7 cells / ml, the culture system can permeate CO 2 gas.

[0030] 3. Fermentation culture

[0031] Put the mother speci...

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Abstract

The invention relates to a method for preparing saponins compounds by fermenting and culturing gen-seng fruits, which comprises the steps: preparing a substrate, sterilizing, culturing seeds, fermenting, separating and extracting. The method for preparing saponins compounds by fermenting and culturing gen-seng fruits is characterized by comprising the following steps: firstly, inoculating wine yeast in a liquid substrate to culture and induce the yeast to form a mother culture with a yeast number of larger than or equal to 10<7> per milliliter; secondly, inoculating the mother culture into the liquid substrate to ferment and culture; carrying out the seed culture and the ferment culture in darkness, at the temperature of between 26 DEG C and 29 DEG C for at least 6 days. Each liter of substrate comprises 600-800ml of gen-seng fruit liquid, 60-80g of glucose and 1-3g of ammonium sulphate in the liquid substrate, and a pH value is from 3 to 4. The method establishes a culture system for producing saponins compounds and has short period, high efficiency and no environmental pollution.

Description

1. Technical background [0001] The invention relates to a method for cell fermentation and cultivation, in particular to a method for preparing saponins by cell fermentation and cultivation of American ginseng fruit juice. 2. Background technology [0002] Humans have a long history of utilizing plant secondary metabolites, and the research on plant secondary metabolites is in full swing today. However, the yield of plant secondary metabolites is limited by the plant yield and the content of secondary metabolites, and the content of some secondary metabolites in nature is very small, which limits the wide application of plant secondary metabolites. With the great progress of biotechnology, people focus on how to use biotechnology to obtain the research methods of plant secondary metabolites. To this end, scientists in related research fields have begun unremitting exploration and experimentation. After decades of research, it has been found that the efficient production of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/645
Inventor 丁之恩严平
Owner ANHUI AGRICULTURAL UNIVERSITY
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