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Oil adjuvant containing panaxoside and preparation method thereof

A technology of ginsenoside and oil adjuvant, applied in the biological field, can solve the problems of large local irritation at the injection site, poor immune effect and the like, and achieve the effect of a simple method

Inactive Publication Date: 2010-03-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of some oil adjuvant vaccines with poor immune effect and large local irritation at the injection site. For this reason, the present invention provides a new adjuvant. While increasing the intensity of cellular and humoral immune responses, it reduces local irritation at the injection site

Method used

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  • Oil adjuvant containing panaxoside and preparation method thereof
  • Oil adjuvant containing panaxoside and preparation method thereof
  • Oil adjuvant containing panaxoside and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Prepare the oil adjuvant containing ginsenoside with domestic No. 10 white oil

[0043] 1. Measure 1000ml of No. 10 white oil (product of Hangzhou Oil Refinery) with a measuring cylinder, and put it in an aluminum pot.

[0044] 2. Heat the white oil with an electric furnace, add 20g of aluminum stearate to the white oil, and stir while adding to make it completely dissolve.

[0045] 3. When the temperature of the white oil drops slightly, add 60ml of Span to the white oil, stir evenly, filter with multiple layers of sterile medical gauze to obtain clear oil, and set aside.

[0046] 4. Weigh 4g of ginseng stem and leaf saponins (product of Jilin Hongjiu Pharmaceutical Co., Ltd.), add it to 10ml of dimethyl sulfoxide (DMSO), mix well, dissolve, and set aside.

[0047] 5. Take 100 microliters of the ginsenoside solution prepared in step 4 and add it to the 1000ml oil adjuvant prepared in step 2, mix well, sterilize at 110°C for 30 minutes, and cool to obtain...

Embodiment 2

[0048] Embodiment 2: French MONTANIDE ISA 206 oil is used to prepare the oil adjuvant containing ginsenoside

[0049] 1. Measure 1000ml MONTANIDE ISA 206 adjuvant (Seppic Company of France) with a graduated cylinder, and put it in an aluminum pot.

[0050] 2. Heat the white oil with an electric furnace, add 16g of aluminum stearate to the white oil, and stir while adding to make it completely dissolve.

[0051] 3. When the temperature of the white oil drops slightly, add 60ml of Span-80 to the white oil, stir evenly, and filter with multiple layers of sterile medical gauze to obtain clear oil for later use.

[0052] 4. Weigh 10g of ginsenosides from stems and leaves of ginseng (product of Jilin Hongjiu Pharmaceutical Co., Ltd.), add it to 10ml of dimethyl sulfoxide (DMSO), mix well, dissolve, and set aside.

[0053] 5. Take 100 microliters of the ginsenoside solution prepared in step 4 and add it to the 1000ml oil adjuvant prepared in step 2, mix well, sterilize at 110°C for ...

Embodiment 3

[0054] Example 3: One of the effects of different contents of ginsenosides in oil seedlings on the immune effect of vaccines

[0055] 1. Preparation of Oil Adjuvant

[0056] (1) Take 1000ml No. 10 white oil (product of Hangzhou Oil Refinery) with a measuring cylinder, and put it in an aluminum pot.

[0057] (2) Heat the white oil with an electric furnace, add 20g of aluminum stearate to the white oil, and stir while adding to make it completely dissolve.

[0058] (3) When the temperature of the white oil drops slightly, add 60ml of Span-80 to the white oil, stir evenly, and filter with multiple layers of sterile medical gauze to obtain a clear oil for later use.

[0059] (4) Weigh 0.5 g of ginseng stem and leaf saponins (GSLS) (product of Jilin Hongjiu Pharmaceutical Co., Ltd.), add it into a test tube containing 10 ml of dimethyl sulfoxide (DMSO), mix well, dissolve, and set aside.

[0060] (5) Get 5 200ml beakers, add 100 milliliters of the oil that step 3 makes respective...

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Abstract

The invention provides an oil adjuvant containing panaxoside and a preparation method thereof. The oil adjuvant is a mixture consisting of panaxoside, adjuvant oil, aluminium stearate and sorbitol mono-oleic acid ester, wherein per milliliter of the oil adjuvant contains 10-100Mug of panaxoside. The preparation method comprises the following steps: heating the oil for preparing the adjuvant to thetemperature of 60-150 DEG C, adding 10-20milligram of aluminium stearate into per milliliter of oil, and mixing evenly; after the temperature of the oil reduces to 50-20 DEG C, adding 5-7% of dehydro-sorbitol mono-oleic acid ester according to volume ratio, stirring evenly, and filtering for standby; and using dimethyl sulfoxide or tween solvent to dissolve the panaxoside into solution; adding 10-100Mug of panaxoside solution into the per milliliter of the prepared standby matter, mixing evenly, and sterilizing for 30min at the temperature of 110 DEG C. Vaccines for immune animals, which is prepared by using oil containing panaxoside as the adjuvant, greatly improve the strength of specificity cells induced by the vaccines and humoral immune response, and reduce local stimulation reactionof injection sites.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a new vaccine adjuvant and a preparation method thereof, in particular to an oil adjuvant containing ginsenoside and a preparation method thereof. Using the adjuvant to prepare vaccines can greatly improve the specificity of vaccine induction. Intensity of sexual immune response and reduction of local irritation at the injection site. Background technique [0002] China is the world's largest breeding country, with a huge number of livestock and poultry, and nearly 900 million pigs, beef cattle, and sheep are slaughtered every year. There are many kinds of animal diseases, and after the scale of large-scale breeding is improved, the diseases are more likely to spread, so the task of animal disease prevention and control is very arduous. Compulsory immunization of livestock and poultry with vaccines is one of the important measures for disease prevention and control. Taking the foot-a...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 胡松华
Owner ZHEJIANG UNIV
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