Method for producing pseudorabies living vaccines by using subculture cell source and product thereof
A technology for subculture cells and pseudorabies, applied in biochemical equipment and methods, medical preparations containing active ingredients, microorganisms, etc., can solve the difficulties in improving vaccine production and efficacy, cell fragments and miscellaneous proteins, and easily cause allergies Response and other issues, to achieve good immune protection, high virus content, improve the effect of vaccine production and quality
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[0021] In order to make the present invention easier to understand, the present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The specific experimental methods not mentioned in the following examples are generally carried out according to conventional experimental methods.
[0022] The production process method of producing pseudorabies live vaccine with passage cell (cell line), concrete steps are as follows:
[0023] (1) Select passage cells as cells for seedling preparation: in this embodiment, pig testis (ST) passage cells are selected, and in the other two examples, pig kidney (PK15) passage cells and pig kidney (IBRS- 2) Subculture cells.
[0024] (2) Passage and culture of cells for seedling preparation: the passaged cells were digested and passaged with EDTA-trypsin cell dispe...
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