Method for producing pseudorabies living vaccines by using subculture cell source and product thereof

Abstract

The invention provides a method for producing pseudorabies living vaccines by using a subculture cell source and a pseudorabies living vaccine product thereof. The method comprises the following steps: culturing pseudorabies virus low-virulent strains by using subculture cells; harvesting the strains to obtain cell culture venom; and then adding a stabilizing agent and an antibiotic into the cell culture venom, and freezing and vacuum-drying the mixture to obtain the pseudorabies living vaccines of the subculture cell source. The subculture cells are subculture cells ST of pig testicle or subculture cells PK15 or IBRS-2 of pig kidney. The method for producing the pseudorabies living vaccines by using the subculture cell source has the advantages of simple and stable production process, easy operation, high virus content, little batch difference and controllable quality, can remarkably improve the yield and quality of the vaccines and reduce the anaphylactic reaction and the like. The pseudorabies living vaccines obtained by using the production method of the invention have good safety and high immune efficacy, and have better immune protection effect on pseudorabies virulent attack.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a method for producing a pseudorabies live vaccine from a subcultured cell source and a product thereof. Background technique [0002] Pseudorabies is an acute infectious disease of various animals caused by pseudorabies virus (PRV). The epidemic spreads throughout the world's major pig-growing countries. Immunization is the main strategy to control pseudorabies. [0003] The cells used in the production of live pseudorabies vaccines in my country are chicken embryo fibroblasts. Due to the difference in raw materials of each batch of chicken embryos, the difference between batches of chicken embryo fibroblast primary cells is relatively large; the titer of toxin production is not high when chicken embryo fibroblasts are used to culture pseudorabies virus, which brings great benefits to the improvement of vaccine production and efficacy. D...

AI Technical Summary

Problems solved by technology

Due to the difference in raw materials of each batch of chicken embryos, the difference between batches of chicken embryo fibroblast primary cells is relatively large; the titer of toxin production is not high when chicken embryo fibroblasts are used to culture pseudorabies virus, which brings great benefits to the improvement of vaccine production and efficacy. Difficult, and there are many cell debris and miscellaneous proteins, which can easily cause allergic reactions