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Antitumor medicament with combination of spirulina polysaccharide and ginkgo-leaf effective components

A technology of spirulina polysaccharides and anti-tumor drugs, which is applied in the field of anti-tumor drugs to achieve a significant anti-tumor effect

Inactive Publication Date: 2010-05-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the anti-tumor research on the combination of spirulina polysaccharides and ginkgo biloba active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: 60 Kunming mice, weighing 20-24 g, half male and half female. They were randomly divided into a spirulina polysaccharide group, a ginkgo flavone group, a ginkgolide group, a combination drug group (spirulina polysaccharide: ginkgo biloba active ingredient=1:1), a positive control group and a blank control group. Mouse right anterior armpit subcutaneously inoculated with S 180 Sarcoma, cell concentration 2×10 7 ~6×10 7 / ml, each mouse was inoculated with 0.2ml cell suspension. It was administered by gavage 24h after inoculation. Each drug group was administered at 200mg / kg.d, the positive control group was given cyclophosphamide solution 20mg / kg.d, and the blank control group was given the same volume of normal saline, once a day for 10 consecutive days. 24h after the last administration, the mice were weighed, the eyeballs were removed and whole blood was taken (1% heparin sodium anticoagulation) to determine the number of peripheral blood leukocytes. The mic...

Embodiment 2

[0015] Example 2: Take human colon cancer HT29 cells in the logarithmic growth phase and adjust the cell concentration to 5×10 4 / mL, add to 96-well culture plate, 200μl per well, set 37℃, 5% CO 2 After culturing for 24 hours in the incubator, they were divided into a combination drug group (spirulina polysaccharide: ginkgo biloba active ingredient = 1:1), spirulina polysaccharide group, ginkgo flavonoid group, and ginkgolide group. The corresponding drugs were added to the culture medium. Drug, the final concentration of the drug in the culture medium is 40μg / mL. Continue to incubate for 72 hours, 4 hours before the end of the culture, add MTT 20μl / well to each well, continue to incubate for 4h, aspirate the supernatant, then add DMSO 200μl / well, shake for 5 minutes with a micro shaker to completely dissolve the dark blue precipitate, use Model 550 porous automatic microplate reader colorimetric (wavelength 570nm), measure the absorbance value, and calculate the growth inhibiti...

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PUM

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Abstract

The invention provides an antitumor medicament with combination of spirulina polysaccharide and ginkgo-leaf effective components, which relates to an antitumor medicament. The antitumor medicament is formed by combining spirulina polysaccharide and ginkgo-leaf effective components, wherein the mass ratio of the spirulina polysaccharide to the ginkgo-leaf effective components is 0.35-1.5:0.35-1.5; the optimal mass ratio of the spirulina polysaccharide to the ginkgo-leaf effective components is 1:1; the spirulina polysaccharide is a water-soluble complex heteropolysaccharide, has the polysaccharide content more than or equal to 93 percent, is formed mainly by connecting D-mannose, D-glucose, D-galactose and glucuronic acid through alpha-type glycosidic bonds, and has the molecular weight of about 12,590 D; and the ginkgo-leaf effective components have the total-flavonoid content more than or equal to 24 percent and the terpene-lactones content more than or equal to 6 percent. The medicament has remarkable tumor-inhibiting effect on S180 sarcoma growing in vivo, as well as human colon cancer HT29 cells, human leukemia HL60 cells, human gastric cancer MGC cells, human cervical carcinoma HeLa cells, human hepatoma 7402 cells and human breast cancer cells growing in vitro.

Description

Technical field: [0001] The invention relates to an anti-tumor drug, in particular to an anti-tumor drug composed of spirulina polysaccharide and ginkgo biloba active ingredients. Background technique: [0002] Tumor diseases are a serious threat to human health, especially malignant tumors. At present, there are no specific therapeutic drugs other than surgical treatment. Although some chemically synthesized anti-tumor drugs used clinically have certain inhibitory and killing effects on tumors, these drugs have also caused great damage to the patient’s body; in recent years, the active ingredients of spirulina polysaccharide and ginkgo biloba have been used. Has been developed into a variety of drugs or health products. There have been many reports on the anti-tumor effect of spirulina polysaccharides, and the vast number of scientific researchers have also conducted a large number of experimental studies. There are few studies and reports on the anti-tumor effect of Ginkgo bi...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61P35/00A61K31/715
Inventor 王清吉葛蔚唐超贾玉辉高玲杨健刘永举
Owner QINGDAO AGRI UNIV
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