Microorganism immobilized carrier and preparation method thereof

A microbial immobilization and carrier technology, which is applied in the direction of immobilization on/in the organic carrier, can solve the problems of weak adsorption force of bacteria, complicated production process of microspheres, low reuse rate, etc., to achieve enhanced activity, The effect of adsorbing more microorganisms and high reuse rate

Active Publication Date: 2010-06-09
GUANGDONG ZHONGKE TIANYUAN NEW ENERGY SCI & TECH
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Problems solved by technology

However, the adsorption force of these magnetic microspheres to the bacteria is weak, the bacteria are easy to fall off, the reuse rate is low, the spheres are easy to accumulate, and the space is occupied. Moreover, the dead bacteria will fall on the surface of the sphere at the bottom after sinking, which affects transmission. quality effect, and the production process of microspheres is complicated and the production cost is high

Method used

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  • Microorganism immobilized carrier and preparation method thereof
  • Microorganism immobilized carrier and preparation method thereof
  • Microorganism immobilized carrier and preparation method thereof

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Embodiment

[0028] refer to figure 1 and 2 , a preparation method of microorganism immobilized carrier, it comprises the following steps:

[0029] 1. Preparation of magnetic PVA membrane carrier:

[0030] Fully dissolve PVA with distilled water, stir at 90°C to make a 10% PVA aqueous solution. 2.5g ferrofluid Fe 3 o 4 Pour into 200mL PVA solution and mix well, mix with the dispersion medium in a round bottom flask, then stir in a 50°C water bath at a speed of 700r / min for 10min, add 3.75mL 25% glutaraldehyde aqueous solution, 3.0mL concentrated hydrochloric acid and 3.0mL 2% sodium alginate aqueous solution, and continue to stir for 1h; pour the solution onto a 200-mesh stainless steel wire mesh covered with nylon mesh (length 240mm×width 80mm), and finally separate the precipitate with a magnetic field, and wash the precipitate with 50% alcohol and distilled water in turn. After repeated washing and drying, the magnetic polyvinyl alcohol film carrier is finally obtained. The disper...

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Abstract

The invention discloses a microorganism immobilized carrier and a preparation method thereof. The immobilized carrier is a helical tube, which is formed by superposing a stainless steel mesh and a nylon mesh and then winding same. A magnetic polyvinylalcohol thin-film layer is covered outside the stainless steel mesh and the nylon mesh. The preparation method for the microorganism immobilized carrier comprises the following steps: preparing the magnetic polyvinylalcohol thin-film layer; modifying and activating the magnetic polyvinylalcohol thin-film layer; preparing the helical tube structure; and immobilizing a microorganism. The microorganism immobilized carrier has the advantages of large surface contact area, multiple absorbed microorganisms, magnetism, simple operation, reduction of production and operation steps, reduction of cost, strong adsorbability, and strong activity and reliability of the carrier, thereby achieving the recycling aim. The occupancy space is reduced; the product yield is improved; and the mass transfer effect cannot be influenced simultaneously.

Description

technical field [0001] The invention relates to the technical field of fermentation industry, in particular to a carrier for immobilizing microorganisms and a preparation method thereof. Background technique [0002] Immobilized cell technology has shown broad development prospects since its inception. Immobilized cells have achieved remarkable results in the ethanol fermentation industry. Compared with traditional fermentation processes, immobilized yeast cells are used to ferment alcohol with fast speed, short cycle, high production capacity, simple process equipment, and easy to achieve continuous and automatic production. Easy to extract and other advantages, and to a certain extent reduce equipment investment and environmental pollution in fermentation plants. [0003] Selecting a suitable immobilized cell carrier is the key to industrialization, and the quality of the carrier depends on the mechanical strength, lifespan, capacity and activity of the embedded microbial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 张宏武陈柏铨傅国英何丹王学伟
Owner GUANGDONG ZHONGKE TIANYUAN NEW ENERGY SCI & TECH
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