Arthrodial cartilage proto micro-carrier

A technology of articular cartilage and microcarriers, which is applied in the field of biomedical tissue engineering, can solve the problems of incomplete separation of cells and microcarriers, affecting the quality of engineered cartilage, and damage to chondrocytes, achieving cheap reagents, avoiding loss, and growing state Good results
CN1868424AInactive Publication Date: 2006-11-29GENERAL HOSPITAL OF PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GENERAL HOSPITAL OF PLA
Publication Date
2006-11-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

A proto-microcarrier for arthrodial cartilage is prepared from fresh joint cartilage through freeze drying, pulverizing, sieving adding trypsin to the particles, oscillating at 37 deg.C for 24-48 hr, washing with distilled water three times, adding TritonX-100, oscillating at 4 deg.C for 48-72 hr, flushing with distilled water for 48 hr, centrifugal separation of deposit, mixing with distilled water, freeze drying, UV irradiating, Co60 irradiation, digestion by trypsin, stirring, pulverizing and washing with distilled water three times.
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Description

Technical field

[0001] The invention relates to a microcarrier, in particular to a preparation method of articular cartilage genic microcarrier and a product prepared by the method, and belongs to the field of biomedical tissue engineering. Background technique

[0002] Tissue engineering technology is the application of biotechnology to prepare tissues identical to the human body in vitro to repair human diseased or defective tissues, also known as regenerative medicine.

[0003] The repair of cartilage defects mainly relies on engineered cartilage tissue. An important condition for the construction of cartilage tissue engineering is the large-scale expansion of chondrocytes in vitro. The use of bioreactor and microcarrier technology to cultivate chondrocytes is a way to efficiently expand chondrocytes. The currently commercially available microcarriers are not only expensive, but also need to digest chondrocytes from the microcarriers during use, which is bound to cause damage ...

Claims

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