Gene activation titanium and preparation method thereof

Abstract

The utility model discloses a gene activation titanium which a titanium body, a pEGFP-hBMP2 plasmid layer and chitosan layer which are sequentially coated on the titanium from interior to exterior with interval to form an activation layer; the gene activation titanium has good biocompatibility and osteoinduction property, can improve the transfection efficiency of host bone cells and effectively regulate and control the gene delivery, and improve the cell vitality, and induce the cell osteogenesis and accordingly enables the titanium body to be well combined with the bone tissues initiatively; the invention also provides the preparation method of the gene activation titanium, which includes that the chitosan layer and the plasmid pEGFP-hBMP2 layer are sequentially precipitated on the surface of the titanium through the static effect between the chitosan molecular and the plasmid DNA; the preparation method not only has simple operation and low cost, but also has simple implementation and ensures the prepared gene activation titanium to effectively induce the conversion from osteogenic cells to osteocyte, and also to be well combined with bone tissues firmly for long time; and the invention is applicable to industrial development.

Description

technical field [0001] The invention relates to a titanium material and a preparation method thereof. Background technique [0002] Titanium is a commonly used medical metal material. Due to its good biocompatibility and mechanical properties, it was used in the medical field as early as the middle of the 20th century, and it began to be widely used as an implant material in the 1970s. With the development of biotechnology and biomedicine, major breakthroughs have been made in the application of titanium materials in human implants, replacement devices and human organs, mainly for dental implants, artificial joints and dental implant brackets, etc. ( Mater Sci Forum, 2003; 416:669-674.) (J Mater Chem 2004; 14:2282-2290). [0003] Titanium is a biologically inert biological material. On the one hand, the biological inertness of the surface of the material endows titanium with good biocompatibility. But on the other hand, this inertness also makes the application of titanium...

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Problems solved by technology

[0005] These methods have improved the biocompatibility and osseointegration ability of titanium materials to a certain extent, but these methods still cannot make titanium materials have good osteoinductive properties, and it is difficult for titanium materials to actively combine with the body and regulate bone cell adhesion, Biological behaviors such as value addition, migration, and differentiation make titanium materials unable to have the ability to osseointegrate well with bone tissue

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