Nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and application thereof
A technology of stenotrophomonas and maltophilia, applied to the nitrogen-fixing stenotrophomonas maltophilia strain C4Y41 and its application fields, can solve the problems of productivity decline, eucalyptus plantation land decline, poor water retention capacity of woodland, etc.
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Embodiment 1
[0023] 1. Isolation and purification of nitrogen-fixing Stenotrophomonas maltophilia strain C4Y41
[0024] The fine roots with nodules were collected from the lateral roots of Podocarpus lobata, rinsed with tap water, sterilized with 75% ethanol for 1 min, and then sterilized with 0.1% mercuric chloride for 5-6 min. Grind the root nodules and fine roots in a sterilized mortar, add a small amount of sterile water, grind well, cover and let stand, and clarify for a while. Take the supernatant and inoculate it on the YMA medium that has been added with Congo red by streaking method, culture at 28°C for about 2 days, select the colonies that do not absorb Congo red, repeat the streaking and screening culture, and then select round, milky white, and producing colonies. Mucoid, neatly bordered colonies. Microscopically, there are flagella, motile, and no spores. Obtain purified strains. The composition and content of the YMA medium are: 0.5g dipotassium hydrogen phosphate, 0.5g m...
Embodiment 2
[0030] Effects of inoculating nitrogen-fixing Stenotrophomonas maltophilia C4Y41 on eucalyptus seedlings by soaking roots
[0031] This experiment was carried out in the nursery of Guangxi State-run Gaofeng Forest Farm located in the suburbs of Nanning.
[0032] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.
[0033] Root soaking method inoculation: before transplanting the above-treated seedlings into the nutrition cup, soak the roots of the seedlings with Stenotrophomonas maltophilia C4Y41 bacteria solution for 20-30 minutes, and then plant them in the nutrition cup. The soil is ye...
Embodiment 3
[0039] Effects of Spray Inoculation of Nitrogen-fixing Stenotrophomonas maltophilia C4Y41 on Eucalyptus Seedlings
[0040] This experiment was carried out in the nursery of Nanning Vegetable Research Institute in the suburb of Nanning.
[0041] The seedlings used in the test came from the Guanglin Eucalyptus grandis No. 9 (GLGU9) clone tissue culture bottle seedlings produced by the Guangxi State-run Gaofeng Forest Farm Tissue Culture Factory. After the tissue culture bottle seedlings are removed from the bottle, the culture medium is cleaned with tap water first, then sterilized with thiophanate-methyl, and the disinfectant on the surface of the seedlings is filtered for later use.
[0042] Spray inoculation method: After the seedlings are transplanted into the nutrition cup, use a watering can to evenly spray the Stenotrophomonas maltophilia C4Y41 bacteria solution on the eucalyptus seedlings planted in the nutrition cup, inoculate a total of 20,000 seedlings, and do not wat...
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