A Chloride Imaging Method of Peripheral Nervous System Based on Laser Confocal Scanning Microscope System

Abstract

The invention discloses a chlorine imaging method of the peripheral nervous system based on a laser confocal scanning microscope system, which comprises the following steps: (1) co-incubation of a single ganglion cell with a chloride ion fluorescent probe MQAE; (2) laser confocal microscope imaging , recording; (3) Correcting the fluorescence value of the chloride ion fluorescent probe MQAE in a single ganglion cell. The main advantages of the present invention are as follows: ① can quantitatively detect the chloride ion concentration of a single ganglion cell in the peripheral nervous system and its variation; ② can visualize the chloride ion concentration in the form of fluorescence to the detected ganglion cells; The probe MQAE is excited under visible light, so the autofluorescence is small and the damage to neurons is small.

Description

Technical field: [0001] The invention belongs to the field of laser confocal imaging, and relates to a peripheral nervous system chloride imaging method based on a laser confocal scanning microscope system. Background technique: [0002] Chloride ion is the most abundant anion in the body. Chloride channels that regulate the concentration of chloride ions inside and outside cells are widely present in the cell membrane and organelle membrane of the body. Chloride channels play an important role in various activities and regulatory processes of cells, such as cell proliferation, apoptosis, regulation of cell excitability, pH regulation, volume regulation and immune response. Therefore, the homeostasis of chloride ions is a necessary condition for maintaining various metabolic activities of cells. Once the homeostasis of chloride ions is disrupted, cells will suffer functional or organic damage or even die. [0003] The laser confocal scanning microscope system is an image d...

AI Technical Summary

Problems solved by technology

However, how to measure the chloride ion concentration for specific cells in the peripheral nervous system (ie, ganglion cells), how to visualize the chloride ion concentration in the cells, and how to monitor the changes of the chloride ion concentration in real time and dynamically have not been reported in China.