Chlorine imaging method of central nervous system based on laser confocal scanning microscope system

Abstract

The invention discloses a chlorine imaging method of a central nervous system based on a laser confocal scanning microscope system, comprising the following steps of: (1) together incubating a brain slice and chloridion fluorescence probe N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE); (2) imaging and recording by using a laser confocal microscope; and (3) correcting the fluorescent value of the MQAE in cells. The invention mainly has the advantages of quantitatively detecting the chlorine ion concentrations in nerve cells and change conditions thereof and visualizing the chlorine ion concentrations in a fluorescent form for the detected nerve cells, and having small self-emitting fluorescence and little damage on the cells because the adopted MQAE is excited in visible light.

Description

Technical field: [0001] The invention belongs to the field of laser confocal imaging, and relates to a central nervous system chlorine imaging method based on a laser confocal scanning microscope system. Background technique: [0002] Chloride ion is the most abundant anion in the body. Chloride channels that regulate the concentration of chloride ions inside and outside cells are widely present in the cell membrane and organelle membrane of the body. Chloride channels play an important role in various activities and regulatory processes of cells, such as cell proliferation, apoptosis, regulation of cell excitability, pH regulation, volume regulation and immune response. Therefore, the homeostasis of chloride ions is a necessary condition for maintaining various metabolic activities of cells. Once the homeostasis of chloride ions is disrupted, cells will suffer functional or organic damage or even die. [0003] The laser confocal scanning microscope system is an image dete...