Supercharge Your Innovation With Domain-Expert AI Agents!

Chlorine imaging method of central nervous system based on laser confocal scanning microscope system

A laser confocal and scanning microscope technology, applied in fluorescence/phosphorescence, material excitation analysis, preparation of test samples, etc., to achieve reliable results, less damage, and less autofluorescence

Active Publication Date: 2010-07-28
FOURTH MILITARY MEDICAL UNIVERSITY
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to measure the concentration of chloride ions in specific cells (mainly neurons) in the nervous system, how to visualize the concentration of chloride ions in cells, and how to monitor the changes of chloride ion concentration in real time and dynamically have not been reported in China.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The chlorine imaging method of the central nervous system based on the laser confocal scanning microscope system follows the following steps: (1) co-incubation of the brain slices with the chloride ion fluorescent probe MQAE; (2) imaging and recording with the laser confocal microscope; (3) calibration of the cells MQAE fluorescence value of the internal chloride ion fluorescent probe.

[0015] 1. Co-incubation of brain slices with chloride ion fluorescent probe MQAE:

[0016] (1) A brain slice with a thickness of 200-300 μm was placed flat on a circular cover glass pre-placed in a 24-well plate for cell culture. Circular coverslips were coated with a polytyrosine solution at a concentration of 1 mg / ml before use to facilitate cell attachment.

[0017] (2) Use a neutral buffer solution fed with a mixed gas, called Krebs-HEPES buffer solution, to wash to remove impurities. It is required to wash 3 times, each time for 5 minutes.

[0018] (3) Add a small amount (100-200...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a chlorine imaging method of a central nervous system based on a laser confocal scanning microscope system, comprising the following steps of: (1) together incubating a brain slice and chloridion fluorescence probe N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE); (2) imaging and recording by using a laser confocal microscope; and (3) correcting the fluorescent value of the MQAE in cells. The invention mainly has the advantages of quantitatively detecting the chlorine ion concentrations in nerve cells and change conditions thereof and visualizing the chlorine ion concentrations in a fluorescent form for the detected nerve cells, and having small self-emitting fluorescence and little damage on the cells because the adopted MQAE is excited in visible light.

Description

Technical field: [0001] The invention belongs to the field of laser confocal imaging, and relates to a central nervous system chlorine imaging method based on a laser confocal scanning microscope system. Background technique: [0002] Chloride ion is the most abundant anion in the body. Chloride channels that regulate the concentration of chloride ions inside and outside cells are widely present in the cell membrane and organelle membrane of the body. Chloride channels play an important role in various activities and regulatory processes of cells, such as cell proliferation, apoptosis, regulation of cell excitability, pH regulation, volume regulation and immune response. Therefore, the homeostasis of chloride ions is a necessary condition for maintaining various metabolic activities of cells. Once the homeostasis of chloride ions is disrupted, cells will suffer functional or organic damage or even die. [0003] The laser confocal scanning microscope system is an image dete...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
Inventor 王亚云杨雁灵季茹李俊杰千年松武胜昔李云庆魏燕燕
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com