Photoperiodic tolerant mutant hd1-3 gene for regulating and controlling flowering time of rice and application thereof

A photoperiod and mutant technology, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problem of low expression

Inactive Publication Date: 2010-08-04
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

[0012] Hd3a is a QTL on the sixth chromosome of rice. It encodes a phosphatidylethanolamine-binding protein and has high homology with FT. It is the flowering of rice Activator, which is highly expressed under short-day conditions but very low under long-day conditions

Method used

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  • Photoperiodic tolerant mutant hd1-3 gene for regulating and controlling flowering time of rice and application thereof
  • Photoperiodic tolerant mutant hd1-3 gene for regulating and controlling flowering time of rice and application thereof
  • Photoperiodic tolerant mutant hd1-3 gene for regulating and controlling flowering time of rice and application thereof

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Embodiment 1

[0026] 1 material

[0027] 1.1 Plant material

[0028] The test materials were japonica rice variety Zhonghua 11 (Oryza sativa L.ssp. Japonica) and its mutant 1f1132. 1f1132 came from the constructed rice T-DNA insertion mutant library, and the genetic background was Zhonghua 11 background. About 500 plants of Zhonghua 11 and 500 plants of 1f1132 were planted in the experimental field of China Rice Research Institute (N30°05′, E119°05′) in the summer of 2007. The sowing time was May 15, May 28, and June respectively. 12th, 23rd June, 10th July and 21st July. The seedlings were transplanted when the seedling age was 20 days, and the heading date was recorded when the heading rate of the main stem of the group reached 50%. The F1 generation of the cross between Zhonghua 11 and 1f1132 was planted in Sanya, Hainan (N18°15′, E109°43′), and the F2 segregant population of the cross was planted in Beijing (N 39°48′, E116°28′). The above materials were kept under routine management ...

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Abstract

The invention relates to a photoperiodic tolerant mutant hd1-3 gene for regulating and controlling the flowering time of rice. The nucleotide sequence of the gene is SEQ ID No.1, and the corresponding hd1-3mRNA sequence of gene is SEQ ID No.2. The invention discovers a photoperiodic tolerant mutant in a constructed rice insertion mutant pool. The mutant provides an extremely good base material for researching the flowering mechanisms of short day plants under the photoperiodic conditions; by the research of the invention, an important functional gene which has specific functions and is used for regulating and controlling the flowering time of the rice is expected to be obtained, which not only provides evidences and the base material for proving the flowering mechanisms of the short day plants under the photoperiodic conditions but also has important practical significance and economic benefit for guiding the breeding of the rice (particularly the breeding of two-series hybrid rice), regulating the growth period of the rice, further improving the yield of the rice and solving the grain problems of the world population. The relevant research provides the theoretical basis for the molecular design of the breeding of the rice and provides references for the production development and the theoretical research of other crops.

Description

[0001] This research was supported by the National Natural Science Foundation of China (30871328) and the Academic Innovation Promotion Program for Young and Middle-aged Teachers of Tianjin Normal University (52X09039, 5RL082). technical field [0002] The invention belongs to the technical field of improving the overall growth and development of rice, and relates to a rice photoperiod insensitive mutant hd1-3 gene and its application. The gene is very important for regulating heading and flowering of rice. Background technique [0003] "Food is paramount to the people", and food plays an important role in the national economy. Rice is one of the most important food crops in the world, and about two-thirds of the world's population takes rice as their main food. Its planting area accounts for 27% of my country's grain area, but its output accounts for 39% of the total grain output. The precise map sequence of rice has been fully completed and published in 2005, which provid...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 栾维江孙宗修
Owner TIANJIN NORMAL UNIVERSITY
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