Unlock instant, AI-driven research and patent intelligence for your innovation.

Single nucleotide polymorphism of human WNK4 genes and application thereof

A single nucleotide polymorphism and gene technology, applied in the fields of molecular biology and medicine, can solve the problem of unproven rs2290041 locus correlation

Inactive Publication Date: 2013-02-13
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been many studies on WNK4 gene polymorphism and essential hypertension, so far, there is no report confirming the correlation between rs2290041 site (mRNA 2902 position C / T) and essential hypertension

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single nucleotide polymorphism of human WNK4 genes and application thereof
  • Single nucleotide polymorphism of human WNK4 genes and application thereof
  • Single nucleotide polymorphism of human WNK4 genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Fluorescent PCR detection

[0037] 1. Experimental materials

[0038] The 7900HT fluorescence quantitative PCR instrument was purchased from ABI Company of the United States, and the polymerase chain reaction solution (TaqMan EXPressMaster Mix) was custom-synthesized by Applied Biosystems (ABI).

[0039] 2. Primer and probe design and synthesis:

[0040] Using the partial sequence of exon 14 of the WNK4 gene as a template, TaqMan primers and probe sites were analyzed using Primer ExpressTM 2.0 software, and custom-synthesized by Applied Biosystems (ABI).

[0041] Primers for detection:

[0042] WNK4 gene rs2290041 upstream primer sequence: 5′-GAGTGCAGAGTGGAGTCTCCCCTAATC-3′

[0043] WNK4 gene rs2290041 downstream primer sequence: 5′-CTCACTGGCTGTGATGACTGTGG-3′

[0044] Fluorescent probes:

[0045] WNK4 gene rs2290041 fluorescent probe 1: 5′-VIC-AGGCTAGAGAGGGGACTA-TAMRA-3′

[0046] WNK4 gene rs2290041 fluorescent probe 2: 5′-FAM-AGGCTAGGGAGGGGACTA-TAMRA-3′

[0047] ...

Embodiment 2

[0059] The rs2290041 site of the WNK4 gene, a susceptibility gene of essential hypertension, was detected by sequencing. 10 cases of each of the above-mentioned hypertension cases and control groups were selected for sequencing to determine the genotype of rs2290041.

[0060] 1. Experimental method

[0061] The above-mentioned fluorescent PCR primers were still used as primers for PCR sequencing, and the amplified products were directly sequenced after purification. The sequencing instrument is ABI's 3130xl genetic analyzer, which is analyzed with sequence analysis5.2 analysis software, and the results can also be viewed with chromas.

[0062] 2. The experimental results showed that the sequencing results of 20 cases were completely consistent with the genotype analysis results of 7900 fluorescent PCR.

[0063] 3. Association analysis of WNK4 gene rs2290041 genotype and hypertension susceptibility

[0064] Comparison of distribution of WNK4 gene rs2290041 in hypertensive pa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biology and medicine, and relates to single nucleotide polymorphism of human WNK4 genes and application thereof, in particular to the single nucleotide polymorphism of the human WNK4 genes and detection of association of the single nucleotide polymorphism of the human WNK4 genes and essential hypertension. The invention also relates to a method for detecting an SNP locus and a kit for detecting susceptibility genes of the hypertension, and is also used for detecting a genotype of the individual WNK4 genes rs2290041 locus and providing reference data for judging whether the risk of the essential hypertension of the individual is greater than that of the general public.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine. A single nucleotide polymorphism involving human WNK4 gene and its use. It specifically relates to the detection of single nucleotide polymorphism (single nucleotide polymorph-phism, SNP) of human WNK4 gene (WNK4 gene) and its correlation with essential hypertension. The invention also relates to a method and a kit for detecting the SNP site. Background technique [0002] Hypertension is a common cardiovascular disease. According to investigations, the incidence rate in developed countries reaches 25%. In recent years, the incidence rate in our country is also on the rise. Taking Beijing as an example, the incidence rate of hypertension has reached as high as 20%. In the elderly population, the prevalence of hypertension is higher. Studies have shown that essential hypertension is a polygenic and multifactorial disease caused by the interaction of environmental factors and gene...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A61K38/45A61P9/12
Inventor 温绍君谢毅李志忠吴海
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV