Autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent as well as culture method and application thereof

A technology of ammonia oxidizing bacteria agent and culture, applied in the field of environmental biology, can solve the problems of global water body eutrophication, ammonia nitrogen pollution, etc., and achieve the effects of resistance to environmental and toxic impacts, strong stability, and improved denitrification effect

Active Publication Date: 2010-09-15
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008]Although some progress has been made in the cultivation of ammonia oxidizing bacteria and the development of bacterial agents, in the face of ammonia nitrogen pollution and the increasing degree of global water eutrophication , in the disc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Preparation of autotrophic and heterotrophic symbiotic ammonia oxidizing bacteria agent

[0037] Medium composition (g / L): NH 4 Cl 0.8, Glucose 0.015, NaHCO 3 1.0, Na 2 HPO 4 10.5, KH 2 PO 4 0.3, MgSO 4 ·7H 2 O 0.5, trace elements 1mL, pH 7.2-7.4, temperature 25-30°C; among them, trace elements (g / 100mL): CuSO 4 ·5H 2 O 0.32, CoCl 2 ·6H 2 O 0.32, (NH4) 6 Mo 7 o 24 4H 2 O 0.24, ZnSO 4 ·7H 2 O 0.44, EDTA1.0, FeSO 4 ·7H 2 O 1.0, MnSO 4 ·H 2 O 0.87.

[0038] The preserved strains were inserted into the above-mentioned culture solution, and activated at 25-30°C for 3 days. Then insert it into fresh culture medium (NH4Cl 0.8g / L) at a ratio of 5%, continue to cultivate for 3-5 days, and collect bacteria with ammonia nitrogen < 10mg / L, that is, liquid autotrophic and heterotrophic symbiotic ammonia oxidizing bacteria agent. The adsorbent used in the solid microbial agent is treated dry wheat bran or rice bran or wood chips, or a mixture th...

Embodiment 2

[0039] Example 2: Removal of ammonia nitrogen in landfill leachate by autotrophic and heterotrophic symbiotic ammonia oxidizing bacteria agent

[0040] Leachate (NH 4 -N: 1810-1910mg / L, COD: 4300-5800mg / L, pH 7.6-7.8) after 5-fold dilution, as a starting solution for a continuous bioreactor (effective volume 1.5L), at a ratio of 5% Add this bacterial agent, temperature 25°C, dissolved oxygen 1.9mg / L, after acclimatization for 5-7 days, use the same diluent as continuous water inflow, the ammonia nitrogen load is kept at 0.10-0.15Kg N / m3.d, and the ammonia nitrogen in the effluent gradually decreases To <10mg N / L, the removal rate of ammonia nitrogen is over 90%. After the ammonia nitrogen in the reactor is stabilized below 10mg / L, gradually replace the influent water with 4 times, 3 times, and 2 times diluted leachate and stock solution. After the ammonia nitrogen in the effluent can be kept below 10mg / L, domestication is completed. The whole stage About 45-50d. During the ...

Embodiment 3

[0041] Example 3: Effect of autotrophic and heterotrophic symbiotic ammonia oxidizing bacteria agent on the removal of ammonia nitrogen in simulated wastewater

[0042] Prepare simulated wastewater, the concentration of ammonia nitrogen is from 100mgN / L to 1000mgN / L, add this bacterial agent at a ratio of 5%, control the temperature at 28.5°C, and cultivate at a medium speed (about 200r / min). Check the pH value every day, when the pH is lower than 6.80, add an appropriate amount of NaHCO 3 Adjust alkalinity. Check the ammonia nitrogen residue every day. The results are shown in Figure 2. In the range of NH4+-N concentration of 100-1000mg / L, the ammonia oxidizing mixed flora grows normally and has high ammonia oxidation activity. The oxidation rate increases with the increase of concentration, and the maximum load can reach 0.150kgN / m3 .d, the residual ammonia concentration is as low as 0-3mg / L.

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Abstract

The invention belongs to the technical field of environmental organisms and particularly relates to an autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent for treating ammonia-nitrogen wastewater as well as a culture method and application thereof. The autotrophic and allotrophic symbiosis ammonia oxidation bacterial agent is prepared by mixing culturing nitrosomonas Nitrosomonas sp.N-1, pseudomonas Pseudomonas sp.He-X, thermomonas Thermomonas haemolytica D-2 and achromobacter Achromobacter xylosoxidans C-1 and is used for treating ammonia-nitrogen wastewater. The invention has the advantages of wide adaptation range, strong stability, environmental and toxic impact resistance and the like.

Description

technical field [0001] The invention belongs to the field of environmental biology technology, and in particular relates to an autotrophic heterotrophic symbiotic ammonia oxidizing bacteria agent for treating ammonia nitrogen wastewater and its application. Background technique [0002] With the development of the global economy, environmental issues have gradually become the focus of people's attention. Protecting the ecological environment and improving people's quality of life have become the consensus of many countries and international organizations. [0003] Ammonia nitrogen is an important pollutant in sewage, and the content of ammonia nitrogen is also an important indicator to measure sewage discharge. With the acceleration of urbanization in our country, the amount of sewage discharged into urban inland rivers and main canals has increased sharply. Especially in recent years, the proportion of industrial wastewater such as printing and dyeing, chemical industry, ph...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/025C12R1/38
Inventor 李大平陶勇何晓红王晓梅
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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