Method for increasing production by compounding microorganism and biologically active agent
A bioactive agent and microbial technology, which is applied in the direction of earthwork drilling, production fluid, wellbore/well components, etc., can solve the problems of slow production effect and general microbial oil recovery effect, so as to improve the effect, enhance oil recovery, long-term effect
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Embodiment 1
[0026] This embodiment includes the following steps:
[0027] 1. First configure the microbial blockage-removing oil-displacing bacterial agent. The microbial plugging-removing oil-displacing bacterial agent is the plugging-removing oil-displacing bacterial agent. The formula includes nutrient agents accounting for 1% of the total weight, and the balance is water and microbial live bacteria. Bacteria is 10 9 cfu / mL, the living microorganisms are aerobic and facultative anaerobic bacteria, and the nutrient contains 500ppm of yeast extract, NH 4 Cl100ppm and K 2 HPO 4 300ppm;
[0028] 2. Configure the bioactive agent. The formula is composed of: 15% rhamnolipid, 30% trehalolipid, 15% alkyl glycoside, and the balance is water;
[0029] 3. Make the microbial plug-removing and oil-displacing bacterial agent prepared in step 1 into a 0.5% aqueous solution, and the bioactive agent prepared in step 2 is also prepared into a 0.5% aqueous solution, and prepare the prepared microbial...
Embodiment 2
[0032] This embodiment includes the following steps:
[0033] 1. First configure the microbial blockage-removing oil-displacing bacterial agent. The microbial plugging-removing oil-displacing bacterial agent is the plugging-removing oil-displacing bacterial agent. The formula includes nutrient agents accounting for 5% of the total weight, and the balance is water and microbial live bacteria. Bacteria is 10 7 cfu / mL, the living microorganisms are aerobic bacteria, and the nutrient contains 50ppm yeast extract, 400ppm peptone, NH 4 Cl300ppm and K 2 HPO 4 200ppm;
[0034] 2. Configure the bioactive agent. The formula is composed of: 20% rhamnolipid, 25% trehalolipid, 10% alkyl glycoside, and the balance is water;
[0035] 3. Make the microbial plug-removing oil-displacing bacterial agent prepared in step 1 into a 2% aqueous solution, and the bioactive agent prepared in step 2 into a 2% aqueous solution, and prepare the prepared microbial plug-removing oil-displacing agent Th...
Embodiment 3
[0038] This embodiment includes the following steps:
[0039] 1. First configure the microbial plugging-removing and oil-displacing bacterial agent. The microbial plugging-removing and oil-displacing bacterial agent is the plugging-removing and oil-displacing bacterial agent. Bacteria is 10 8 cfu / mL, the microbial live bacteria are facultative anaerobic bacteria, and the nutritional agent contains yeast extract 300ppm, peptone 200ppm, NH 4 Cl600ppm and K 2 HPO 4 50ppm;
[0040] 2. Configure the bioactive agent. The formula is composed of: 25% rhamnolipid, 20% trehalolipid, 20% alkyl glycoside and the balance is water.
[0041]3. Make the microbial plug-removing oil-displacing bacterial agent prepared in step 1 into a 1% aqueous solution, and the bioactive agent prepared in step 2 also into a 1% aqueous solution, and prepare the prepared microbial plug-removing oil-displacing agent The aqueous solution of the bacterial agent and the aqueous solution of the bioactive agent ...
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