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Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof

A technology of Streptomyces griseus and alfalfa, applied in the field of microorganisms, can solve the problems that the research results cannot really serve agricultural production, and the screening results are unreliable.

Inactive Publication Date: 2011-12-07
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, mostly only adopt flat plate antibacterial test method (such as plate confrontation method, inhibition zone method, Oxford cup method, tube and plate method, dig block method, agar block method or filter paper sheet method), spore germination method etc. The in vitro screening model screens active strains, and the screening results are unreliable, making the follow-up research results unable to truly serve agricultural production

Method used

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  • Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof
  • Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof
  • Streptomyces griseoflavus for resisting alfalfa diseases and screening method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, isolation and identification of bacterial strain

[0041] 1. Separation

[0042] Gao's No. 1 medium: agar 15g, soluble starch 20g, NaCl 0.5g, KNO 3 1g, K 2 HPO 4 ·3H 2 O0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, distilled water 1000mL, pH7.2-7.4.

[0043] PDA agar medium: Potato (peeled) 200g, sucrose (or glucose) 20g, distilled water 1000mL, natural pH.

[0044] Fusarium solani (Fusarium solani); (Cao Lixia, Zhao Cunhu, Bai Quanjiang, et al. Research on the pathogen of alfalfa root rot in central Inner Mongolia (English) [J]. North China Agricultural Science, 2008, 23(6): 105-107. )

[0045] (1) The pure culture of actinomycetes was isolated from the soil

[0046] Strains were isolated from soil samples collected from Mu Us sandy land in Ordos City, Inner Mongolia by dilution plate separation method.

[0047] The soil was collected, and the collected soil samples were naturally air-dried, sieved, and weighed. The main actinomycetes gro...

Embodiment 2

[0186] Embodiment 2, the application of bacterial strain NMG6-3-9

[0187] 1. Determination of antibacterial spectrum of strain NMG6-3-9:

[0188] PDA agar medium: potato (peeled) 200g, sucrose (or glucose) 20g, distilled water 1000mL, pH natural.

[0189] Inoculate the actinomycete strain NMG6-3-9 on the Gaoshi No. 1 agar plate and culture it at 28°C for 5 days to obtain a bacterial lawn; punch the bacterial lawn into a bacterial cake with a diameter of 5mm, and place the bacterial cake In the center of the PDA plate, stick the side with mycelium on the surface of the medium, culture at 30°C for 3 days, then inoculate the same size of target bacteria cakes around the strain NMG6-3-9, culture at 28°C until the target Bacteria until the culture dish is overgrown. The diameter of the bacteriostatic (bacteria lysing) zone was measured by the cross method.

[0190] The above-mentioned plate antibacterial test adopts the conventional plate confrontation method, the Oxford cup me...

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Abstract

The invention discloses streptomyces griseoflavus for resisting alfalfa diseases and a screening method thereof. The strain is streptomyces griseoflavus NMG 6-3-9, and the preservation number of the streptomyces griseoflavus is CGMCC No. 3441. The strain of the invention has the excellent antagonistic effect on pathogenic bacteria of alfalfa root rot, can be used for the biological control of alfalfa root rot and other multiple plant diseases, and has the wide application prospect in the field of biological control of diseases. The streptomyces griseoflavus establishes the foundation for the biological control of alfalfa root rot, and provides the scientific basis for the development and application of biocontrol actinomyces preparations of diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a streptomyces griseoflavus resistant to alfalfa disease and a screening method thereof. Background technique [0002] Since the 1950s, extensive use of chemical pesticides has caused pathogenic bacteria to develop resistance to chemical pesticides, high residues have caused environmental pollution, and chemical pesticides have caused toxicity to humans and animals, which has accelerated people's search for new, safe and effective ways to prevent and control plant diseases. Among the various measures to prevent and control plant diseases, biological control has the advantages of being relatively safe to the environment, ecology and human health. Therefore, countries all over the world attach great importance to the development and utilization of biological control microorganism research. Biocontrol microorganisms are not easy to cause drug resistance of pathoge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04A01N63/02A01P3/00A01P1/00C12R1/465
Inventor 徐林波陈红印张礼生刘爱萍狄彩霞刘雅学周玉雷荆瑞勇高书晶王宁塔娜闫丽英刘星李鹏赵海霞石雅琴李薇
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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