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Streptoseomycin and preparation method and application thereof

A technology of streptomycin and streptomyces, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., to achieve the effects of guaranteed source, overcoming drug resistance, and simple process

Inactive Publication Date: 2012-06-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no report on the isolation of antibiotics from Streptomyces seoulensis

Method used

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  • Streptoseomycin and preparation method and application thereof
  • Streptoseomycin and preparation method and application thereof
  • Streptoseomycin and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and purification of Streptomyces seoulensis of marine origin

[0029] Dissect the prawn (Penaeus chinensis) under aseptic conditions, disinfect the surface of the removed prawn intestinal tract in 75% alcohol for 2 minutes, rinse with sterile water for 3 times, add a small amount of sterile water and grind it in a sterile mortar. Gradiently dilute the polishing solution with sterile water to 10 -1 , 10 -2 , 10 -3 , 10 -4 , take 0.2 milliliters of each gradient dilution solution and smear it on Gao's No. 1 medium (soluble starch 20g, KNO 3 1g, NaCl 0.5g, K 2 HPO 4 ·3H 2 O 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 (00.01g, 1L distilled water), place 28 ℃ of incubators to cultivate, after the bacterial colony grows out, pick the spores to purify and cultivate, and obtain the actinomycetes of shrimp intestinal tract, which is identified as Streptomyces Seoul (Streptomyces seoul) by Institute of Microbiology, Chinese Academy of Sciences ( Str...

Embodiment 2

[0030] Example 2: Liquid fermentation of marine-derived Streptomyces seoulensis

[0031] Activate Streptomyces seoulensis from the sea, inoculate fresh thallus blocks into 1000mL Erlenmeyer flasks, each bottle contains 400mL of Gaussian medium, inoculate 5-6 bottles on a shaker, at 150rpm, 28°C Cultivate under certain conditions for 5 days as seed liquid, then inoculate 20 mL of seed liquid into a 1000 mL conical flask containing 400 mL of Gaussian or Martin medium, and ferment for 8 days at 150 rpm and 20-30°C.

Embodiment 3

[0032] Embodiment 3: the extraction and separation of Seoul streptomycin (streptoseomycin)

[0033] The fermented liquid obtained in Example 2 was filtered through gauze, the filtrate was extracted with ethyl acetate, concentrated and dried in vacuo to obtain black crude extract F1. Suspend the crude extract F1 in water, sequentially extract with petroleum ether and ethyl acetate, and concentrate the obtained ethyl acetate extract to obtain extract F2, carry out silica gel column chromatography on extract F2, and use 4 times the volume of chloroform successively, Chloroform: methanol = 100: 1, chloroform: methanol = 100: 2, chloroform: methanol = 100: 4, elute, concentrate 100: 4 (chloroform: methanol) section to obtain black extract F3, and then extract F3 Separation of Sephadex LH-20 (eluent chloroform: methanol = 1: 1) combined with the eluate of the green fluorescent part to obtain a light yellow extract F4, and the extract F4 was separated with a reversed-phase column ODS...

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Abstract

The invention belongs to the biological pharmacy technical field, in particular relates to streptoseomycin extracted from marine actinomycete streptomyces seoulensis liquid fermentation as well as preparation method and application thereof. Streptoseomycin is obtained by separation, purification and fermentation on streptomyces seoulensis from ocean. Streptoseomycin has strong inhibition function on anaerobe and has no toxicity on cell, thus streptoseomycin can be taken as lead compound of antibiotic production and can be applied in preparation of antibiotic medicine.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to streptoseomycin extracted from liquid fermented product of marine actinomyces (Streptomyces seoulensis) and its preparation method and application. Background technique [0002] Although the secondary metabolites of microorganisms have been widely studied, the metabolites of some special environment microorganisms have not attracted enough attention. The particularity of the marine environment has created the unique metabolic mode and metabolites of marine actinomycetes. In recent years, people continue to search for antibiotics with new mechanisms of action from actinomycetes in various marine environments. In addition to antibiotics, marine actinomycetes can also produce inhibitors of various enzymes, and the diversity of marine actinomycetes provides a rich source of secondary metabolites. [0003] With the widespread use of antibiotics, the drug resistance of pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/18C07D491/22A61K31/407A61P31/04C12R1/465
Inventor 谭仁祥宋勇春乔红运梅亚宁刘敏吴琦
Owner NANJING UNIV
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