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Small-sized seed germinating method

A seed and petri dish technology, applied in the agricultural field, can solve the problems of difficult to achieve aseptic operation, germination is not closed, etc., and achieve the effect of easy operation

Inactive Publication Date: 2012-01-11
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination of the current paper bed is not closed, and aseptic operation is difficult to achieve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The method for this small seed germination is carried out as follows:

[0014] a. Take one petri dish with a diameter of 9 cm and two filter papers with a diameter of 11 cm, insert the filter paper into it according to the shape of the petri dish and make the center of the filter paper coincide with the center of the petri dish; then

[0015] b. Take out the filter paper and put it upside down in the petri dish. In order to give the seeds the space needed for germination, press down the surface of the filter paper properly, and control the distance between the filter paper surface and the bottom of the petri dish to be 4mm and then

[0016] c. Add water with a water surface height of 3 mm from the wall of the petri dish, then place the seeds evenly on the surface of the filter paper, cover the lid, seal it with a parafilm, and put it into the incubator.

Embodiment 2

[0018] The method for this small seed germination is carried out as follows:

[0019] a. Take one petri dish with a diameter of 9 cm and four filter papers with a diameter of 11 cm, insert the filter paper into it according to the shape of the petri dish and make the center of the filter paper coincide with the center of the petri dish; then

[0020] b. Take out the filter paper and put it upside down in the petri dish. In order to give the seeds the space needed for germination, press down the surface of the filter paper properly, and control the distance between the surface of the filter paper and the bottom of the petri dish to be 7mm; then

[0021] c. Add water with a water surface height of 1 mm from the wall of the petri dish, then place the seeds evenly on the surface of the filter paper, cover the lid, seal it with a parafilm, and put it into the incubator.

Embodiment 3

[0023] The method for this small seed germination is carried out as follows:

[0024] a. Take one petri dish with a diameter of 9 cm and three filter papers with a diameter of 11 cm, insert the filter paper into it according to the shape of the petri dish and make the center of the filter paper coincide with the center of the petri dish; then

[0025] b. Take out the filter paper and put it upside down in the petri dish. In order to give the seeds the space needed for germination, press down the surface of the filter paper properly, and control the distance between the surface of the filter paper and the bottom of the petri dish to be 5mm; then

[0026] c. Add water with a water surface height of 2 mm from the wall of the petri dish, then place the seeds evenly on the surface of the filter paper, cover the lid, seal it with a parafilm, and put it into the incubator.

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Abstract

The invention provides a small-sized seed germinating method. The method comprises the following steps of: a, taking a culture dish and a plurality of pieces of filter paper with the diameter greater than that of the culture dish, padding the filter paper into the culture dish according to the shape of the culture dish, and superposing the centers of the filter paper and the culture dish; b, taking the filter paper out, backing off the filter paper into the culture dish, properly pressing the surface of the filter paper downwards, and controlling the surface of the filter paper not to contactthe bottom of the culture dish; and c, adding a proper amount of water into the culture dish from the wall of the culture dish, putting seeds on the surface of the filter paper uniformly, covering the culture dish, sealing the culture dish by using a sealing film, and putting the culture dish into an incubator.

Description

Technical field: [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for germination of small seeds. Background technique: [0002] Seed germination test is an extremely important index for judging seed quality in agriculture, forestry, horticulture and scientific research and production. Accurate test results have important practical significance for determining the appropriate sowing rate and ensuring full seedlings. It is also one of the main indicators for our national gene bank to measure the quality of various plant seeds. At present, there are three commonly used germination beds for determining the germination rate of seeds: paper bed, sand bed and soil. Paper beds are very popular because they are fast, convenient and easy to operate. However, the study found that some small seeds (such as "Piananthoma") or seeds with a long germination time (such as "Cistanche"), as well as very small seeds (such as "hand gins...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
Inventor 陈庆亮王志芬单成钢倪大鹏朱彦威
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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