Methods for extracting papain and superoxide dismutase (SOD) crude enzyme from comman floweringquince fruit

Abstract

The invention discloses methods for extracting papain and a superoxide dismutase (SOD) complex enzyme from comman floweringquince fruit. The method for extracting the papain from the comman floweringquince fruit comprises the following steps of: washing and peeling fresh comman floweringquince fruit, removing seeds from the comman floweringquince fruit, and uniformly grinding; performing ultrasonic extraction; freezing and centrifuging; crudely filtering supernate; performing ultrafiltration; and freezing and drying to obtain the papain. The method for extracting the SOD enzyme from the comman floweringquince fruit comprises the following steps of: washing and peeling the fresh comman floweringquince fruit and removing seeds; adding extraction buffering solution into the obtained product and uniformly grinding; freezing and centrifuging; filtering the supernate; settling with acetone; performing ultrafiltration; and freezing and drying to obtain the SOD complex enzyme. The methods have the advantages that: the simple operation is adopted in the processes for extracting the papain and the SOD, processing cost is relatively low and the obtained enzyme is high in purity and activity.

Description

1. Technical background [0001] The invention belongs to the field of bioengineering, in particular to a technique for extracting papain and SOD enzyme. 2. Background technology [0002] Papain (Papain, E C 3.4.22.2), also known as papain, is a class of thiol proteases. Widely present in the fruits, leaves, stems and roots of papaya (Carica papaya), among which the most abundant content is in immature milk [71] . It has the characteristics of good thermal stability, natural hygiene and safety, and high enzyme activity, so it is widely used in food, daily chemical, feed, medicine, textile and leather industries. And because of its good stability, it has a good degradation effect on various proteins, and its proteolysis ability is strong, so it has been widely used in many industries. In the food industry: for beer clarification, meat tenderization, fish processing and feed additives; in the industrial industry: for cocoon degumming, gelatin manufacturing, leather depilation...

AI Technical Summary

Problems solved by technology

At present, the extraction of papain mainly adopts chemical methods. On the one hand, chemical methods require a lot of process conditions, and the operation process is cumbersome, time-consuming and energy-consuming; on the other hand, a large amount of chemical reagents are required, and the cost is very high.

The traditional extraction method of papain has serious defects: the harvesting method of papaya green fruit milk is backward, the sanitary conditions are poor, the milk loss is large, the browning is serious, and the enzyme activity is low

After determination, the fruit of Xuan papaya contains high SOD activity, but so far no in-depth research on its extraction process has been carried out. After searching, no report about extracting SOD enzyme from Xuan papaya fruit has been found.