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Low-temperature emulsification process of animal oil emulsion inactivated vaccines

A technology of inactivated vaccine and process method, applied in the field of vaccine preparation, can solve the problem of easy destruction of vaccine active antigen, and achieve the effects of ideal immune efficacy, stable product quality, and increased yield

Inactive Publication Date: 2011-02-23
SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a low-temperature emulsification process for veterinary oil emulsion inactivated vaccines, to overcome the problem that the active antigen of the vaccine is easily destroyed in the existing emulsification process, so that the yield of the vaccine is high and the quality stability is good

Method used

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  • Low-temperature emulsification process of animal oil emulsion inactivated vaccines
  • Low-temperature emulsification process of animal oil emulsion inactivated vaccines
  • Low-temperature emulsification process of animal oil emulsion inactivated vaccines

Examples

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Embodiment 1

[0013] Such as figure 1 Shown, be the avian influenza (H9 hypotype F strain) inactivated vaccine emulsification process method, comprise the steps: 1), carry out the preparation of water phase in water phase tank and carry out the preparation of oil phase in oil phase tank, its The preparation method is the same as the preparation method of the prior art. After the preparation is completed, the water phase and the oil phase are cooled to 5-8°C; 2), the emulsification tank is pre-cooled, so that the temperature inside the tank is 5-8°C; 3) 1. Put the cooled water phase and oil phase together into the emulsification tank for pre-mixing, and keep the temperature in the emulsification tank at 5-8°C; 4) Perform high-speed stirring and emulsification in the emulsification tank to make the water droplets further broken and uniform Dispersed in the oil phase, during the emulsification process, keep the temperature in the emulsification tank at 5-8°C, and use cold water at a temperatur...

Embodiment 2

[0023] Also used as figure 1 The low-temperature emulsification process shown in the production of chicken Newcastle disease, avian influenza (H9 subtype F strain) dual inactivated vaccine comprises the following steps: 1), the preparation of the water phase is carried out in the water phase tank and the preparation of the water phase is carried out in the oil phase tank. The preparation of the oil phase, its preparation method is the same as the preparation method of the prior art. After the preparation is completed, the water phase and the oil phase are cooled to 5-8 ° C; 2), the emulsification tank is pre-cooled, so that the temperature in the tank is 5 -8°C; 3), add the cooled water phase and oil phase together into the emulsification tank for pre-mixing, keep the temperature in the emulsification tank at 5-8°C; 4), carry out high-speed stirring and emulsification in the emulsification tank, The water droplets are further broken and evenly dispersed in the oil phase. Durin...

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Abstract

The invention relates to a low-temperature emulsification process of animal oil emulsion inactivated vaccines, which belongs to the field of vaccine preparation methods. The low-temperature emulsification process comprises the following steps of: 1) preparing a water phase in a water-phase tank and preparing an oil phase in an oil-phase tank, and cooling the water phase and the oil phase to 5 to 8 DEG C after the preparation; 2) pre-cooling an emulsification tank to control the temperature in the tank body to be 5 to 8 DEG C; 3) adding the cooled water phase and oil phase into the emulsification tank together for premixing and keeping the temperature in the emulsification tank at 5 to 8 DEG C; and 4) stirring at a high speed in the emulsification tank for emulsification to make water drops further broken and uniformly dispersed in the oil phase, wherein the temperature in the emulsification tank is kept at 5 to 8 DEG C during emulsification. When the process is used for preparing the inactivated vaccines, damage to an active antigen is small, yield is high, and product stability is stable. The process makes the different adjuvant types of inactivated vaccines achieve the same effect and can be used for preparing various animal oil emulsion inactivated vaccines.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to an emulsification method for preparing an oil emulsion inactivated vaccine. Background technique [0002] The preparation of the existing veterinary oil emulsion inactivated vaccine mainly includes the following steps: firstly prepare the water phase in the water phase tank and prepare the oil phase in the oil phase tank, and then put the water phase and the oil phase together Add it into the emulsification tank for pre-mixing. The stirring speed of the pre-mixing in the emulsification tank is low. The pre-mixing makes the water evenly dispersed into the oil. However, due to the low speed, the combination between water and oil is not tight enough, and it is necessary to use High-speed stirring is used for further emulsification, so that the water droplets are further broken and evenly dispersed in the oil phase, and an emulsified inactivated vaccine with relatively stable phys...

Claims

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Application Information

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IPC IPC(8): A61J3/00A61K39/00A61K9/107
Inventor 陈清周玉双刘雷
Owner SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
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