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Method for preparing refined celery extract

A technology for extracts and celery, which is applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve the problems of low purity of extracts and poor application effect, and achieve the effect of optimizing traditional processes

Inactive Publication Date: 2012-01-04
隋焕平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Macroporous adsorption resin is a kind of organic polymer adsorbent, which has the principle of adsorption and molecular sieve separation, and has good adsorption and separation performance for organic compounds. It has been widely used in the extraction and separation of chemical components in plants. The ingredients are effective, but some extracts have low purity, such as: the extraction purity of flavonoids in the prior art is only 15% to 30%, and the application effect is not very good

Method used

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specific Embodiment approach 1

[0007] Specific embodiment one: In this embodiment, a method for preparing the refined extract of celery is implemented according to the following steps: one, the celery is cleaned, cut into sections, frozen at -20~-30°C for 12~18h, then at 10~30°C Melt at 3000-10000r / min for homogenization, add 2-30g cellulase at pH 4.0-5.0, enzymolyze at 35-65°C for 30-120min, then add absolute ethanol, The volume ratio of absolute ethanol to the solution after enzymolysis is 4-6:1, reflux at 75-95°C for 2-3 hours, cool to 2-10°C, filter, then collect the filtrate, and concentrate the filtrate under reduced pressure to 50 The crude extract of celery is obtained when the relative density is 1.25 to 1.35 under the condition of ℃; two, the crude extract of celery obtained in step 1 is obtained by ethanol dissolution with a volume fraction of 50%, and the volume ratio of ethanol and crude extract of celery is 0.5 during dissolving ~1:1, then add in AB-8 macroporous resin column for adsorption, t...

specific Embodiment approach 2

[0008] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, freezing for 13-17 hours. Others are the same as the first embodiment.

specific Embodiment approach 3

[0009] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, freezing for 14-16 hours in step 1. Others are the same as the first embodiment.

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Abstract

The invention discloses a method for preparing a refined celery extract, relates to a method for preparing an extract from chemical ingredients in a plant and solves the problems of low ingredient purity and poor application effect of a flavonoid in the conventional celery extract. The method for preparing the refined celery extract comprises the following steps of: 1, preparing a crude celery extract; 2, adsorbing the prepared celery extract by using AB-8 macro-porous resin; and 3, drying the celery extract in vacuum so as to obtain the refined celery extract. In the refined celery extract, a flavonoid ingredient has high purity and good application effect. The method is widely applied to the extraction and separation of the chemical ingredients in the plant and is an effective method for separating and purifying plant ingredients.

Description

technical field [0001] The invention relates to a method for preparing extracts of chemical components in plants. Background technique [0002] Celery (Apium graveolens L.) is a 1-year or 2-year-old herb of the Umbelliferae family. Studies have confirmed that celery is rich in flavonoids. Pharmacologically, it has the effects of lowering blood pressure, calming the nerves, lowering blood sugar and blood fat, softening blood vessels, and enhancing immunity. Flavonoids have the functions of scavenging free radicals, anti-aging and preventing and treating senile diseases. Fully extracting the flavonoids in celery is the key to rational utilization of celery resources. Macroporous adsorption resin is a kind of organic polymer adsorbent, which has the principle of adsorption and molecular sieve separation, and has good adsorption and separation performance for organic compounds. It has been widely used in the extraction and separation of chemical components in plants. The met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/23A61P9/12A61P25/20A61P3/10A61P3/06A61P9/00A61P37/04A61P39/06
Inventor 隋焕平
Owner 隋焕平
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