Method for studying dielectric property of cell by using ultrasonic pulse dynamic electricity
A technology of dielectric properties and ultrasonic pulses, applied in the direction of measuring electricity, measuring electrical variables, measuring devices, etc., can solve the problem of not being able to study the ultrasonic biological effects of cells in real time
Inactive Publication Date: 2013-07-10
XI AN JIAOTONG UNIV
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0006] The purpose of the present invention is to overcome the deficiency that the existing method for researching the dielectric properties of cells cannot study the ultrasonic biological effects of cells in real time under the action of ultrasound, and to provide a method for using ultrasonic pulse electrokinetics to study the dielectric properties of cells. Electrokinetic theoretical model, by continuously adjusting the functional state of ultrasound, so as to realize the study of real-time ultrasound biological effects under the action of ultrasound
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0053] The present invention is described in further detail below in conjunction with accompanying drawing:
[0054] The method of the present invention using ultrasonic pulse electrokinetics to study the dielectric properties of cells is specifically carried out according to the following steps:
[0055] 1) Establish cell unit model
[0056] The macroscopically detected electrokinetic potential results from the balance of electrokinetic interactions between microscopic electrogravity and viscous shear in the electrical double layer of the cell. Therefore, assuming that a cell with a diameter of a and uniformly distributed negative charges on its surface is surrounded by an ion-permeable polymer electrolyte double layer of thickness d, this whole is called a cell unit, which is described as an inner diameter It is a, spherical soft colloidal particles with an outer diameter of b=a+d; assuming that the cell unit model is a fixed charge aggregate with a valence of Z, and its ou...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a method for studying the dielectric property of the cell by using ultrasonic pulse dynammic electricity, comprising the following steps: establishing a cell unit model, preparing a cell suspension, detecting CVI (cell vibration current), calculating the CVI and analyzing the dielectric property of the cell according to the CVI detection result. When the CVI is detected, the ultrasonic pulse with the angular frequency omega is made to act on both the cell suspension and normal saline, and the total current TVI in the cell suspension and the ion vibration current in thenormal saline are detected. The method for studying the dielectric property of the cell by using ultrasonic pulse dynammic electricity can be used to study the dielectric property of the cell by making ultrasonic pulse act on the cell and accurately detecting the change of charges on the surface of the cell so as to achieve the purpose of studying the ultrasonic biological effect of the cell. Thedevice applied to the method for studying the dielectric property of the cell by using ultrasonic pulse dynammic electricity has simple structure, and the method for studying the dielectric property of the cell by using ultrasonic pulse dynammic electricity has good reproducibility and can not cause any damage to the cell.
Description
Technical field [0001] The invention belongs to the field of basic theoretical research of biological cells. It is a method that uses ultrasonic pulse electrical to study cell dielectric characteristics. This method uses ultrasonic pulse as a sound probe based on the theoretical model of suspended cells to study cell dielectric from the charge level.Features can be used to study the surface of cell membrane on the surface of the cell membrane, especially in the action of ultrasonic effects under the action of ultrasound. Background technique [0002] A certain dose of ultrasonic radiation can cause a variety of biological effects in different levels of biological systems.So far, in many cases, the physical mechanism of ultrasonic biological effects is still unclear, and there is no suitable way to study the ultrasonic biological effect in real time.At present, the research methods of cellular dielectric characteristics mainly include cell electrical, electrochemical impedance met...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01R31/00
Inventor 钟力生成林张跃于钦学施毅舟
Owner XI AN JIAOTONG UNIV