Device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse

Abstract

The invention discloses a device for dynamically and electrically researching dielectric property of cells by using ultrasonic pulse. The device comprises a computer, an acquisition card and a support with a vertical liquid accommodating cavity; an ultrasonic transducer is arranged in the liquid accommodating cavity of the support; an ultrasonic reflection preventing sound absorption material is filled between the lower end of the ultrasonic transducer and the bottom of the liquid accommodating cavity; a cavity for accommodating a cell suspension sample is positioned above the ultrasonic transducer in the liquid accommodating cavity; a sampling electrode in liquid level contact with the cell suspension sample is arranged at the upper end opening of the liquid accommodating cavity, the sampling electrode is connected with a micro current detection circuit, and the micro current detection circuit is connected to the computer through the acquisition card; and two electrodes of the ultrasonic transducer are connected with ultrasonic signal generating sources. The device can realize research of real-time ultrasonic biological effect under the action of ultrasonic according to a dynamicelectric theoretical model of cell suspension by continuously adjusting the functional state of the ultrasonic; and the device has a simple structure and good repeatability, is easy to operate, and has no damage to the cells.

Description

technical field [0001] The invention belongs to the field of biological cell basic theory research, and relates to a device for studying the dielectric properties of cells, especially a device for studying the dielectric properties of cells by using ultrasonic pulse electrokinetics. The electric theory model studies the dielectric properties of cells from the charge level, which can be used to study the charging characteristics of the cell membrane surface, especially for the real-time study of the ultrasonic biological effects of cells under the action of ultrasound. Background technique [0002] A certain dose of ultrasonic radiation can cause a variety of biological effects in different levels of biological systems. So far, in many cases, the physical mechanism of ultrasonic biological effects is still unclear, and there is no suitable method to study the process of ultrasonic biological effects in real time. At present, the research methods for the dielectric properties...

AI Technical Summary

Problems solved by technology

According to the Debye-Bueche model and the acoustic theory of the soft colloidal particle suspension, the total current (TVI) in the cell suspension can be expressed as the sum of the ion vibration current (IVI) and the cell vibration current (CVI), as shown in the following formula, However, no relevant theoretical models and implementation methods for the actual cell suspension electrokinetic theory have been proposed.

Images